Matches in SemOpenAlex for { <https://semopenalex.org/work/W1994102999> ?p ?o ?g. }
- W1994102999 endingPage "428" @default.
- W1994102999 startingPage "415" @default.
- W1994102999 abstract "Differential scanning fluorimetry (DSF) is successfully used as a high-throughput screening method for the analysis of the protein melting temperature (Tm) in the development of therapeutic monoclonal antibody (MAb) formulations. Typically, surfactants are utilized in MAb formulations as a stabilizer, but the commonly applied polarity-sensitive dye SYPRO® Orange shows bright fluorescence in the presence of micelles, concealing the signal of protein unfolding. Studying various MAb formulations containing polysorbate 20, polysorbate 80, or poloxamer 188 (PX 188), the molecular rotor probe 4-(dicyanovinyl)julolidine (DCVJ) was investigated. Although limited to higher MAb concentrations, DCVJ enabled the determination of Tm in many formulations where SYPRO® Orange failed. It is important to note that careful background correction of placebo formulations is essential for the precise determination of Tm and especially Tm onset. Thermal shifts of Tm1 (lowest observed thermal transition) indicating stabilizing or destabilizing effects of pH or excipient were in good agreement across all tested formulations and correlated well with differential scanning calorimetry measurements. Additionally, the micellization temperature of PX 188 was confirmed, which leads to a nonproteinous transition. With this new method, it is possible to apply DSF during the development of therapeutic proteins in surfactant-containing formulations." @default.
- W1994102999 created "2016-06-24" @default.
- W1994102999 creator A5016601141 @default.
- W1994102999 creator A5070571988 @default.
- W1994102999 date "2013-02-01" @default.
- W1994102999 modified "2023-09-26" @default.
- W1994102999 title "High-Throughput Melting-Temperature Analysis of a Monoclonal Antibody by Differential Scanning Fluorimetry in the Presence of Surfactants" @default.
- W1994102999 cites W1963719513 @default.
- W1994102999 cites W1965524375 @default.
- W1994102999 cites W1967693680 @default.
- W1994102999 cites W1971212417 @default.
- W1994102999 cites W1977149004 @default.
- W1994102999 cites W1977802438 @default.
- W1994102999 cites W1987307858 @default.
- W1994102999 cites W1989570165 @default.
- W1994102999 cites W1998833577 @default.
- W1994102999 cites W2000106654 @default.
- W1994102999 cites W2003511105 @default.
- W1994102999 cites W2008630616 @default.
- W1994102999 cites W2018263847 @default.
- W1994102999 cites W2035813163 @default.
- W1994102999 cites W2039377812 @default.
- W1994102999 cites W2040230742 @default.
- W1994102999 cites W2047132220 @default.
- W1994102999 cites W2054328264 @default.
- W1994102999 cites W2054609339 @default.
- W1994102999 cites W2062214242 @default.
- W1994102999 cites W2066262772 @default.
- W1994102999 cites W2071920844 @default.
- W1994102999 cites W2072398283 @default.
- W1994102999 cites W2076100371 @default.
- W1994102999 cites W2077203674 @default.
- W1994102999 cites W2078439031 @default.
- W1994102999 cites W2079029484 @default.
- W1994102999 cites W2081411678 @default.
- W1994102999 cites W2081787960 @default.
- W1994102999 cites W2082877775 @default.
- W1994102999 cites W2083412871 @default.
- W1994102999 cites W2091229935 @default.
- W1994102999 cites W2094731907 @default.
- W1994102999 cites W2104717900 @default.
- W1994102999 cites W2108305553 @default.
- W1994102999 cites W2109732625 @default.
- W1994102999 cites W2111418206 @default.
- W1994102999 cites W2111877521 @default.
- W1994102999 cites W2118732577 @default.
- W1994102999 cites W2127061933 @default.
- W1994102999 cites W2131851892 @default.
- W1994102999 cites W2133569069 @default.
- W1994102999 cites W2133741696 @default.
- W1994102999 cites W2134625581 @default.
- W1994102999 cites W2143550120 @default.
- W1994102999 cites W2145275398 @default.
- W1994102999 cites W2152335348 @default.
- W1994102999 cites W2158750984 @default.
- W1994102999 cites W2159351791 @default.
- W1994102999 cites W2159840467 @default.
- W1994102999 doi "https://doi.org/10.1002/jps.23405" @default.
- W1994102999 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23212746" @default.
- W1994102999 hasPublicationYear "2013" @default.
- W1994102999 type Work @default.
- W1994102999 sameAs 1994102999 @default.
- W1994102999 citedByCount "68" @default.
- W1994102999 countsByYear W19941029992013 @default.
- W1994102999 countsByYear W19941029992014 @default.
- W1994102999 countsByYear W19941029992015 @default.
- W1994102999 countsByYear W19941029992016 @default.
- W1994102999 countsByYear W19941029992017 @default.
- W1994102999 countsByYear W19941029992018 @default.
- W1994102999 countsByYear W19941029992019 @default.
- W1994102999 countsByYear W19941029992020 @default.
- W1994102999 countsByYear W19941029992021 @default.
- W1994102999 countsByYear W19941029992022 @default.
- W1994102999 countsByYear W19941029992023 @default.
- W1994102999 crossrefType "journal-article" @default.
- W1994102999 hasAuthorship W1994102999A5016601141 @default.
- W1994102999 hasAuthorship W1994102999A5070571988 @default.
- W1994102999 hasConcept C101335993 @default.
- W1994102999 hasConcept C11268172 @default.
- W1994102999 hasConcept C113196181 @default.
- W1994102999 hasConcept C121332964 @default.
- W1994102999 hasConcept C158711907 @default.
- W1994102999 hasConcept C15920480 @default.
- W1994102999 hasConcept C159654299 @default.
- W1994102999 hasConcept C178790620 @default.
- W1994102999 hasConcept C184651966 @default.
- W1994102999 hasConcept C185592680 @default.
- W1994102999 hasConcept C203014093 @default.
- W1994102999 hasConcept C2777239854 @default.
- W1994102999 hasConcept C2777897440 @default.
- W1994102999 hasConcept C2994048982 @default.
- W1994102999 hasConcept C39519442 @default.
- W1994102999 hasConcept C43617362 @default.
- W1994102999 hasConcept C521977710 @default.
- W1994102999 hasConcept C542903549 @default.
- W1994102999 hasConcept C55493867 @default.
- W1994102999 hasConcept C58226133 @default.
- W1994102999 hasConcept C62520636 @default.