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- W1994129003 abstract "“Browning” of leaf extracts from Mentha species prevents accurate analysis of the proteins by disc-electrophoresis. Seventeen reagents were compared to determine minimum browning and maximum extraction of proteins; pre-extraction of phenols with aqueous acetones was also investigated. 2-Mercaptoethanol, sodium mercaptoacetate, sodium ascorbate, l(+)-cysteine, sodium hydrosulfite, dithioerythritol, sodium, N,N-diethyldithiocarbamate, and potassium metabisulfite were demonstrated to be effective for extraction of protein, but all these reagents except 2-mercaptoethanol interfered with disc-electrophoresis. Pre-extraction of phenols from fresh leaves with 80 per cent acetone prepared with an alkaline buffer or distilled water was also demonstrated to be effective for extraction of protein, but the disc-electrophoretic bands were less distinct than those obtained with 2-mercaptoethanol." @default.
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- W1994129003 date "1970-07-01" @default.
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- W1994129003 title "Extraction of leaf proteins of mentha (labiatae) for disc-electrophoresis" @default.
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- W1994129003 doi "https://doi.org/10.1016/s0031-9422(00)85253-x" @default.
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