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• W1994129029 endingPage "1475" @default.
• W1994129029 startingPage "1465" @default.
• W1994129029 abstract "Diatoms are dominant organisms in phototrophic biofilms in aquatic habitats. They produce copious amounts of extracellular polymeric substances (EPS), which mainly consist of carbohydrates and traces of proteins and glycoproteins. This study focuses on the characterization of EPS from a total of 14 diatoms belonging to the six genera Achnanthes, Cymbella , Fragilaria , Punctastriata , Staurosira, and Pseudostaurosira , all of which were isolated from epilithic biofilms of the littoral zone of Lake Constance. EPS from all isolates were extracted by a sequential extraction procedure resulting in five different fractions. The monosaccharide composition of each fraction was analyzed by HPLC equipped with a pulse amperiometric detector, yielding results similar to those obtained by probing the EPS structures with monomer‐specific fluorophore‐linked lectins. Significant differences in carbohydrate composition occurred in the different fractions of single isolates. Most of the diatom isolates in our study form chain‐like colonies in which the cells are attached to each other by intercellular pads. Here we demonstrate that these pads can be dissolved in hot bicarbonate and that they show a heterogeneous composition of monosaccharides in contrast to other fractions, which mostly were dominated by one or two monosaccharides. Principal component analysis indicates a correlation between carbohydrate composition of EPS fractions and the phylogenetic relationship of the respective species, indicating that EPS analyses under defined culture conditions may support taxonomic analyses." @default.
• W1994129029 created "2016-06-24" @default.
• W1994129029 creator A5004756190 @default.
• W1994129029 creator A5010254855 @default.
• W1994129029 date "2008-12-01" @default.
• W1994129029 modified "2023-10-18" @default.
• W1994129029 title "THE COMPLEX EXTRACELLULAR POLYSACCHARIDES OF MAINLY CHAIN-FORMING FRESHWATER DIATOM SPECIES FROM EPILITHIC BIOFILMS¹" @default.
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• W1994129029 doi "https://doi.org/10.1111/j.1529-8817.2008.00609.x" @default.
• W1994129029 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27039861" @default.
• W1994129029 hasPublicationYear "2008" @default.
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