Matches in SemOpenAlex for { <https://semopenalex.org/work/W1994132314> ?p ?o ?g. }
- W1994132314 endingPage "e447" @default.
- W1994132314 startingPage "e447" @default.
- W1994132314 abstract "A challenging goal in biology is to understand how the principal cellular functions are integrated so that cells achieve viability and optimal fitness in a wide range of nutritional conditions.We report here a tight link between glycolysis and DNA synthesis. The link, discovered during an analysis of suppressors of thermosensitive replication mutants in bacterium Bacillus subtilis, is very strong as some metabolic alterations fully restore viability to replication mutants in which a lethal arrest of DNA synthesis otherwise occurs at a high, restrictive, temperature. Full restoration of viability by such alterations was limited to cells with mutations in three elongation factors (the lagging strand DnaE polymerase, the primase and the helicase) out of a large set of thermosensitive mutants affected in most of the replication proteins. Restoration of viability resulted, at least in part, from maintenance of replication protein activity at high temperature. Physiological studies suggested that this restoration depended on the activity of the three-carbon part of the glycolysis/gluconeogenesis pathway and occurred in both glycolytic and gluconeogenic regimens. Restoration took place abruptly over a narrow range of expression of genes in the three-carbon part of glycolysis. However, the absolute value of this range varied greatly with the allele in question. Finally, restoration of cell viability did not appear to be the result of a decrease in growth rate or an induction of major stress responses.Our findings provide the first evidence for a genetic system that connects DNA chain elongation to glycolysis. Its role may be to modulate some aspect of DNA synthesis in response to the energy provided by the environment and the underlying mechanism is discussed. It is proposed that related systems are ubiquitous." @default.
- W1994132314 created "2016-06-24" @default.
- W1994132314 creator A5001144818 @default.
- W1994132314 creator A5003565422 @default.
- W1994132314 creator A5018121693 @default.
- W1994132314 creator A5032518519 @default.
- W1994132314 creator A5035338313 @default.
- W1994132314 creator A5041356288 @default.
- W1994132314 creator A5051095340 @default.
- W1994132314 creator A5051164464 @default.
- W1994132314 creator A5053446528 @default.
- W1994132314 creator A5058015886 @default.
- W1994132314 creator A5087410187 @default.
- W1994132314 creator A5090132131 @default.
- W1994132314 date "2007-05-16" @default.
- W1994132314 modified "2023-10-10" @default.
- W1994132314 title "Genetic Evidence for a Link Between Glycolysis and DNA Replication" @default.
- W1994132314 cites W1411612488 @default.
- W1994132314 cites W1479875019 @default.
- W1994132314 cites W1497274060 @default.
- W1994132314 cites W1511524557 @default.
- W1994132314 cites W1513345255 @default.
- W1994132314 cites W1543201946 @default.
- W1994132314 cites W1579835890 @default.
- W1994132314 cites W1639658090 @default.
- W1994132314 cites W1838792009 @default.
- W1994132314 cites W1966688525 @default.
- W1994132314 cites W1972868028 @default.
- W1994132314 cites W1972926920 @default.
- W1994132314 cites W1975473030 @default.
- W1994132314 cites W1980964194 @default.
- W1994132314 cites W1993993762 @default.
- W1994132314 cites W2000216662 @default.
- W1994132314 cites W2008549086 @default.
- W1994132314 cites W2010128870 @default.
- W1994132314 cites W2011456814 @default.
- W1994132314 cites W2015631023 @default.
- W1994132314 cites W2020271217 @default.
- W1994132314 cites W2021743090 @default.
- W1994132314 cites W2024400556 @default.
- W1994132314 cites W2028357761 @default.
- W1994132314 cites W2030113713 @default.
- W1994132314 cites W2032457228 @default.
- W1994132314 cites W2035385414 @default.
- W1994132314 cites W2035478230 @default.
- W1994132314 cites W2036799174 @default.
- W1994132314 cites W2046928699 @default.
- W1994132314 cites W2047228870 @default.
- W1994132314 cites W2053359084 @default.
- W1994132314 cites W2062175081 @default.
- W1994132314 cites W2062345761 @default.
- W1994132314 cites W2066886643 @default.
- W1994132314 cites W2070925652 @default.
- W1994132314 cites W2071289410 @default.
- W1994132314 cites W2074465394 @default.
- W1994132314 cites W2085519916 @default.
- W1994132314 cites W2087434357 @default.
- W1994132314 cites W2090047431 @default.
- W1994132314 cites W2090632954 @default.
- W1994132314 cites W2091608178 @default.
- W1994132314 cites W2091682844 @default.
- W1994132314 cites W2098169274 @default.
- W1994132314 cites W2098461709 @default.
- W1994132314 cites W2100882223 @default.
- W1994132314 cites W2101304175 @default.
- W1994132314 cites W2101322984 @default.
- W1994132314 cites W2102822826 @default.
- W1994132314 cites W2104539744 @default.
- W1994132314 cites W2106130068 @default.
- W1994132314 cites W2108091116 @default.
- W1994132314 cites W2110851412 @default.
- W1994132314 cites W2110902629 @default.
- W1994132314 cites W2114563758 @default.
- W1994132314 cites W2114809602 @default.
- W1994132314 cites W2119344497 @default.
- W1994132314 cites W2121165657 @default.
- W1994132314 cites W2123925153 @default.
- W1994132314 cites W2124610014 @default.
- W1994132314 cites W2124703740 @default.
- W1994132314 cites W2126909443 @default.
- W1994132314 cites W2129523337 @default.
- W1994132314 cites W2129727440 @default.
- W1994132314 cites W2130503287 @default.
- W1994132314 cites W2130661537 @default.
- W1994132314 cites W2133041897 @default.
- W1994132314 cites W2135202729 @default.
- W1994132314 cites W2136134333 @default.
- W1994132314 cites W2138081694 @default.
- W1994132314 cites W2139885113 @default.
- W1994132314 cites W2142437744 @default.
- W1994132314 cites W2142837955 @default.
- W1994132314 cites W2146538795 @default.
- W1994132314 cites W2147220368 @default.
- W1994132314 cites W2152714161 @default.
- W1994132314 cites W2155422793 @default.
- W1994132314 cites W2156320330 @default.
- W1994132314 cites W2161630640 @default.
- W1994132314 cites W2164997719 @default.