FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1994172337> ?p ?o ?g. }

• W1994172337 endingPage "966" @default.
• W1994172337 startingPage "955" @default.
• W1994172337 abstract "Cisplatin is a potent anticancer agent forming intrastrand-crosslinks in DNA. The efficacy of cisplatin in chemotherapy can be limited by the development of tumor resistances such as elevated DNA repair or damage tolerance. In Escherichia coli, cisplatin treatment causes induction of the SOS regulon resulting in elevated levels of DNA Pol II, DNA Pol IV, DNA Pol V, the cell division inhibitor SfiA (SulA), homologous recombination (HR) and DNA repair. In this work, the roles of Pol II and HR in facilitating resistance of E. coli to cisplatin are studied. SOS induction levels were measured by beta-galactosidase assays in cisplatin-treated and untreated E. coli PQ30 that has the lacZ gene fused to the sfiA promoter. Comparative studies were carried out with derivatives of PQ30 constructed by P1 transduction that have transposon insertions in the polB gene, the recB gene blocking the RecBCD pathway of HR and genes of the RecFOR pathway of HR. Resistance of E. coli strains to cisplatin as determined by plating experiments decreased in the following order: parent PQ30 strain, polB > recO, recR, recF > recB. Both the RecBCD and RecFOR pathways of HR are important for survival when E. coli is exposed to cisplatin, because treatment of double mutants deficient in both pathways reduced colony forming ability to 37% in 6-9min in comparison to 39-120min for single mutants. Pol II and RecF appear to function in two distinct pathways to initiate replication blocked due to damage caused by cisplatin because function of Pol II was required for survival in mutants deficient in the RecFOR pathway after 2h of cisplatin treatment. In contrast, Pol II was not required for survival in recB mutants. SOS induction was delayed in RecFOR deficient mutants but occurred at high levels in the recB mutant soon after cisplatin treatment in a RecFOR-dependent way. An SfiA independent, DNA damage dependent pathway is apparently responsible for the filamentous cells observed after cisplatin or MMC treatments of these SfiA defective strains." @default.
• W1994172337 created "2016-06-24" @default.
• W1994172337 creator A5033444925 @default.
• W1994172337 creator A5087177018 @default.
• W1994172337 date "2002-11-01" @default.
• W1994172337 modified "2023-09-26" @default.
• W1994172337 title "Survival and SOS induction in cisplatin-treated Escherichia coli deficient in Pol II, RecBCD and RecFOR functions" @default.
• W1994172337 cites W1485044984 @default.
• W1994172337 cites W1497274060 @default.
• W1994172337 cites W1507507742 @default.
• W1994172337 cites W1520746855 @default.
• W1994172337 cites W1520909561 @default.
• W1994172337 cites W1547905266 @default.
• W1994172337 cites W1571718539 @default.
• W1994172337 cites W1804945000 @default.
• W1994172337 cites W1893642377 @default.
• W1994172337 cites W1967804444 @default.
• W1994172337 cites W1975222291 @default.
• W1994172337 cites W1976056964 @default.
• W1994172337 cites W1976453250 @default.
• W1994172337 cites W1977477496 @default.
• W1994172337 cites W1980411311 @default.
• W1994172337 cites W1982692787 @default.
• W1994172337 cites W1987940280 @default.
• W1994172337 cites W1988094074 @default.
• W1994172337 cites W1990259081 @default.
• W1994172337 cites W1992739680 @default.
• W1994172337 cites W1993057099 @default.
• W1994172337 cites W1996903808 @default.
• W1994172337 cites W1997478704 @default.
• W1994172337 cites W2012108057 @default.
• W1994172337 cites W2012549906 @default.
• W1994172337 cites W2012578976 @default.
• W1994172337 cites W2021183094 @default.
• W1994172337 cites W2028940434 @default.
• W1994172337 cites W2036166614 @default.
• W1994172337 cites W2036682748 @default.
• W1994172337 cites W2048549484 @default.
• W1994172337 cites W2054017383 @default.
• W1994172337 cites W2057954230 @default.
• W1994172337 cites W2058231848 @default.
• W1994172337 cites W2061643429 @default.
• W1994172337 cites W2068899404 @default.
• W1994172337 cites W2071850307 @default.
• W1994172337 cites W2073657752 @default.
• W1994172337 cites W2074069247 @default.
• W1994172337 cites W2075554927 @default.
• W1994172337 cites W2082545983 @default.
• W1994172337 cites W2093065152 @default.
• W1994172337 cites W2094047668 @default.
• W1994172337 cites W2101859984 @default.
• W1994172337 cites W2109836043 @default.
• W1994172337 cites W2114235371 @default.
• W1994172337 cites W2114989573 @default.
• W1994172337 cites W2121001380 @default.
• W1994172337 cites W2130442072 @default.
• W1994172337 cites W2133290100 @default.
• W1994172337 cites W2136970762 @default.
• W1994172337 cites W2140784150 @default.
• W1994172337 cites W2153418663 @default.
• W1994172337 cites W2162699077 @default.
• W1994172337 cites W2167738884 @default.
• W1994172337 cites W2168211826 @default.
• W1994172337 cites W2318792136 @default.
• W1994172337 cites W67746198 @default.
• W1994172337 doi "https://doi.org/10.1016/s1568-7864(02)00147-7" @default.
• W1994172337 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12531023" @default.
• W1994172337 hasPublicationYear "2002" @default.
• W1994172337 type Work @default.
• W1994172337 sameAs 1994172337 @default.
• W1994172337 citedByCount "15" @default.
• W1994172337 countsByYear W19941723372012 @default.
• W1994172337 countsByYear W19941723372018 @default.
• W1994172337 countsByYear W19941723372020 @default.
• W1994172337 countsByYear W19941723372022 @default.
• W1994172337 crossrefType "journal-article" @default.
• W1994172337 hasAuthorship W1994172337A5033444925 @default.
• W1994172337 hasAuthorship W1994172337A5087177018 @default.
• W1994172337 hasConcept C104317684 @default.
• W1994172337 hasConcept C134935766 @default.
• W1994172337 hasConcept C143425029 @default.
• W1994172337 hasConcept C153911025 @default.
• W1994172337 hasConcept C174742784 @default.
• W1994172337 hasConcept C2776694085 @default.
• W1994172337 hasConcept C2778239845 @default.
• W1994172337 hasConcept C2780852648 @default.
• W1994172337 hasConcept C54355233 @default.
• W1994172337 hasConcept C547475151 @default.
• W1994172337 hasConcept C552990157 @default.
• W1994172337 hasConcept C86803240 @default.
• W1994172337 hasConceptScore W1994172337C104317684 @default.
• W1994172337 hasConceptScore W1994172337C134935766 @default.
• W1994172337 hasConceptScore W1994172337C143425029 @default.
• W1994172337 hasConceptScore W1994172337C153911025 @default.
• W1994172337 hasConceptScore W1994172337C174742784 @default.
• W1994172337 hasConceptScore W1994172337C2776694085 @default.
• W1994172337 hasConceptScore W1994172337C2778239845 @default.
• W1994172337 hasConceptScore W1994172337C2780852648 @default.

• next