FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1994174953> ?p ?o ?g. }

• W1994174953 endingPage "3877" @default.
• W1994174953 startingPage "3866" @default.
• W1994174953 abstract "Abstract Objective To explore the potential involvement of the chemokine system in synoviocyte‐mediated tissue destruction in rheumatoid arthritis (RA), we studied the expression profile of chemokine receptors and their function in the migration, proliferation, and matrix metalloproteinase (MMP) production of cultured fibroblast‐like synoviocytes (FLS) from RA patients. Methods The presence of CC and CXC chemokine receptors on cultured FLS was studied at the messenger RNA (mRNA) level by reverse transcriptase–polymerase chain reaction and at the cell surface expression level by flow cytometry. Variations in cytosolic calcium influx induced by chemokine stimulation were assessed by flow cytometry on Fura Red–preloaded FLS. Two‐compartment transwell chambers were used for FLS chemotaxis assays. Cell growth was measured by a fluorescence‐based proliferation assay. Gelatinase and collagenase activities were determined by a fibril degradation assay and zymography. Results FLS constitutively expressed the receptors CCR2, CCR5, CXCR3, and CXCR4, both at the cell surface and mRNA levels, but failed to express CCR3 and CCR6. Significant intracytosolic calcium influx was observed on FLS challenged with monocyte chemotactic protein 1 (MCP‐1), stromal cell–derived factor 1α (SDF‐1α), and interferon‐inducible protein 10 (IP‐10). Stimulation with MCP‐1, SDF‐1α, IP‐10, and monokine induced by interferon‐γ enhanced the migration and proliferation of FLS. These chemokines, in addition to RANTES, increased in a dose‐ and time‐dependent manner the gelatinase and collagenase activities in cell‐free supernatants of cultured FLS. Interestingly, the chemokine‐mediated up‐regulation of MMP activities was significantly abrogated by the presence of anti–interleukin‐1β, but not anti–tumor necrosis factor α, blocking antibodies. Conclusion These data suggest that through modulation of the migration, proliferation, and MMP production by FLS, the chemokine system may play a more direct role in the destructive phase of RA than is currently suspected, and thus emphasize the relevance of chemokines and their receptors as potential therapeutic targets in this disease." @default.
• W1994174953 created "2016-06-24" @default.
• W1994174953 creator A5004363203 @default.
• W1994174953 creator A5011908954 @default.
• W1994174953 creator A5020886257 @default.
• W1994174953 creator A5027413583 @default.
• W1994174953 creator A5046925974 @default.
• W1994174953 creator A5051065833 @default.
• W1994174953 creator A5076189703 @default.
• W1994174953 date "2004-12-01" @default.
• W1994174953 modified "2023-10-18" @default.
• W1994174953 title "CC and CXC chemokine receptors mediate migration, proliferation, and matrix metalloproteinase production by fibroblast-like synoviocytes from rheumatoid arthritis patients" @default.
• W1994174953 cites W1511309136 @default.
• W1994174953 cites W1543575007 @default.
• W1994174953 cites W1543705865 @default.
• W1994174953 cites W1561950821 @default.
• W1994174953 cites W1571810458 @default.
• W1994174953 cites W1602835962 @default.
• W1994174953 cites W1606902694 @default.
• W1994174953 cites W1804074798 @default.
• W1994174953 cites W1870510950 @default.
• W1994174953 cites W1887877287 @default.
• W1994174953 cites W1915685928 @default.
• W1994174953 cites W1990336673 @default.
• W1994174953 cites W1998256351 @default.
• W1994174953 cites W1998605540 @default.
• W1994174953 cites W1998866269 @default.
• W1994174953 cites W2003284223 @default.
• W1994174953 cites W2007451263 @default.
• W1994174953 cites W2010042266 @default.
• W1994174953 cites W2010715043 @default.
• W1994174953 cites W2011548322 @default.
• W1994174953 cites W2012827463 @default.
• W1994174953 cites W2018706434 @default.
• W1994174953 cites W2019709132 @default.
• W1994174953 cites W2023266494 @default.
• W1994174953 cites W2025878623 @default.
• W1994174953 cites W2032226212 @default.
• W1994174953 cites W2033809811 @default.
• W1994174953 cites W2039664649 @default.
• W1994174953 cites W2041161248 @default.
• W1994174953 cites W2046412495 @default.
• W1994174953 cites W2052346247 @default.
• W1994174953 cites W2057097380 @default.
• W1994174953 cites W2061860330 @default.
• W1994174953 cites W2066904629 @default.
• W1994174953 cites W2074438676 @default.
• W1994174953 cites W2076342409 @default.
• W1994174953 cites W2076538283 @default.
• W1994174953 cites W2079199816 @default.
• W1994174953 cites W2091215793 @default.
• W1994174953 cites W2094570628 @default.
• W1994174953 cites W2102859558 @default.
• W1994174953 cites W2110535065 @default.
• W1994174953 cites W2115706633 @default.
• W1994174953 cites W2121718569 @default.
• W1994174953 cites W2123884480 @default.
• W1994174953 cites W2134486617 @default.
• W1994174953 cites W2152348310 @default.
• W1994174953 cites W2154344438 @default.
• W1994174953 cites W2157542668 @default.
• W1994174953 cites W2170601120 @default.
• W1994174953 cites W2171197664 @default.
• W1994174953 cites W2172005240 @default.
• W1994174953 cites W2344697123 @default.
• W1994174953 doi "https://doi.org/10.1002/art.20615" @default.
• W1994174953 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15593223" @default.
• W1994174953 hasPublicationYear "2004" @default.
• W1994174953 type Work @default.
• W1994174953 sameAs 1994174953 @default.
• W1994174953 citedByCount "158" @default.
• W1994174953 countsByYear W19941749532012 @default.
• W1994174953 countsByYear W19941749532013 @default.
• W1994174953 countsByYear W19941749532014 @default.
• W1994174953 countsByYear W19941749532015 @default.
• W1994174953 countsByYear W19941749532016 @default.
• W1994174953 countsByYear W19941749532017 @default.
• W1994174953 countsByYear W19941749532018 @default.
• W1994174953 countsByYear W19941749532019 @default.
• W1994174953 countsByYear W19941749532020 @default.
• W1994174953 countsByYear W19941749532021 @default.
• W1994174953 countsByYear W19941749532022 @default.
• W1994174953 countsByYear W19941749532023 @default.
• W1994174953 crossrefType "journal-article" @default.
• W1994174953 hasAuthorship W1994174953A5004363203 @default.
• W1994174953 hasAuthorship W1994174953A5011908954 @default.
• W1994174953 hasAuthorship W1994174953A5020886257 @default.
• W1994174953 hasAuthorship W1994174953A5027413583 @default.
• W1994174953 hasAuthorship W1994174953A5046925974 @default.
• W1994174953 hasAuthorship W1994174953A5051065833 @default.
• W1994174953 hasAuthorship W1994174953A5076189703 @default.
• W1994174953 hasConcept C12823836 @default.
• W1994174953 hasConcept C13373296 @default.
• W1994174953 hasConcept C153911025 @default.
• W1994174953 hasConcept C170493617 @default.
• W1994174953 hasConcept C179661763 @default.
• W1994174953 hasConcept C185592680 @default.
• W1994174953 hasConcept C29146819 @default.

• next