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- W1994187534 abstract "Expression from the Bacillus subtilis nrg-21 locus Increases 26-fold during nitrogen-limited growth. The DNA corresponding to this locus was cloned and sequenced. The nucleotide sequence revealed a gene that could encode a protein with sequence similarity to the Escherichia coll gamma-aminobutyric acid (GABA) permease. A transposon insertion in this locus eliminated the uptake of GABA and severely inhibited the utilization of GABA as a nitrogen source. Primer extension analysis revealed that the B. subtilis gabP gene was transcribed from two overlapping promoters. Transcription from the P1 promoter was repressed during growth in the presence of amino acids. The product of the codY gene proved to be required for this repression. Transcription from the P2 promoter increased during nitrogen-limited growth and was dependent upon the product of the tnrA gene. Deletion analysis revealed that activation of the P2 promoter during nitrogen-limited growth requires a nucleotide sequence located upstream of its -35 region. Regulation of gabP expression by the CodY and TnrA regulatory systems, which respond to different physiological signals, allows for a wide range of gabP expression during growth on various nitrogen sources." @default.
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- W1994187534 date "1996-11-01" @default.
- W1994187534 modified "2023-10-13" @default.
- W1994187534 title "<b>Expression of the <i>Bacillus subtilis gabP</i> gene is regulated independently in response to nitrogen and amino acid availability</b>" @default.
- W1994187534 doi "https://doi.org/10.1046/j.1365-2958.1996.d01-1720.x" @default.
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