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- W1994226103 abstract "Isolated pollen grains were obtained from precultured anthers of a japonica rice cultivar Taipei 309. They were cultured in modified Gamborg's B5 liquid medium (E10) containing various amino acids. It was observed that only large pollen grains, 50–58 μm in diameter with thin, light pink colored cell walls seemed to develop into pollen embryos. Division frequency of pollen increased rapidly during the first 10 days of culture in the four media tested. Culture in E10 liquid medium with 1 mM proline yielded the highest division frequency of 3.9% and plating efficiency of 3.5%. After a 14-day culture period, microcalli derived from the pollen grains were transferred into Murashige and Skoog (MS) liquid medium supplemented with 1.0 mg/l kinetin, 1.0 mg/l naphthalene acetic acid and 20 mg/l abscisic acid (MSA20). For plant regeneration, growing calli were transferred onto Murashige and Skoog (MS) semisolid medium containing 1 mg/l kinetin and 1 mg/l naphthalene acetic acid (MSYo). Out of 57 plants, 16 were haploids and the others were homozygous diploids." @default.
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- W1994226103 date "1988-01-01" @default.
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- W1994226103 title "Callus formation and plant regeneration in isolated pollen culture of rice (Oryza sativa L. cv. Taipei 309)" @default.
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- W1994226103 doi "https://doi.org/10.1016/0168-9452(88)90014-3" @default.
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