FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1994249027> ?p ?o ?g. }

• W1994249027 endingPage "205" @default.
• W1994249027 startingPage "197" @default.
• W1994249027 abstract "The structure of purified rhodopsin was investigated by steady-state resonance energy transfer and fluorescence quenching techniques: (1) Fluorescence parameters and relative distances between rhodopsin sites labeled with fluorescent probes and the endogenous chromophore 11-cis-retinal were measured in micellar detergent solution and in reconstituted phospholipid vesicles. (2) The accessibility of the labeled rhodopsin sites in reconstituted vesicles to N-methyl- and N-benzylpicolinium was studied in the dark and subsequent to rhodopsin bleaching. Fluorescent-labeled rhodopsin was affinity purified in octyl glucoside from rod outer segments which were previously reacted with either the sulfhydryl-specific reagents, pyrenylmaleimide or monobromobimane, or reagents specific to amino groups, dansyl chloride or fluorescein isothiocyanate. The purified protein was recombined with phospholipids, and vesicles were formed by detergent dialysis. All four fluorophores appear to react greater than or equal to 30 A away from the endogenous chromophore as estimated from the efficiency of energy transfer and presumably probe rhodopsin domains exposed at the membrane surface. The maximal fraction of quenchable fluorescence and the mean quenching constant were determined in dark and bleached vesicles: bleaching did not affect the quenching of the fluorophores attached to sulfhydryl groups but markedly decreased the quenching constants of the fluorophores coupled to amino groups. The apparent collisional rate constant decreased by 20- and 4-fold for dansyl and fluorescein, respectively. The results suggest that bleaching reduced the accessibility of these sites which, in turn, may reflect light-induced displacements of rhodopsin domains at the membrane surface. Such structural changes may regulate rhodopsin-rhodopsin as well as rhodopsin-enzyme interactions." @default.
• W1994249027 created "2016-06-24" @default.
• W1994249027 creator A5047434116 @default.
• W1994249027 creator A5086815695 @default.
• W1994249027 date "1983-01-04" @default.
• W1994249027 modified "2023-10-14" @default.
• W1994249027 title "Rhodopsin in reconstituted phospholipid vesicles. 1. Structural parameters and light-induced conformational changes detected by resonance energy transfer and quenching" @default.
• W1994249027 cites W141032628 @default.
• W1994249027 cites W1489585315 @default.
• W1994249027 cites W1521958691 @default.
• W1994249027 cites W1572544720 @default.
• W1994249027 cites W1575474218 @default.
• W1994249027 cites W1594934722 @default.
• W1994249027 cites W1963713347 @default.
• W1994249027 cites W1965613189 @default.
• W1994249027 cites W1966969615 @default.
• W1994249027 cites W1967788950 @default.
• W1994249027 cites W1970028705 @default.
• W1994249027 cites W1972673432 @default.
• W1994249027 cites W1974617220 @default.
• W1994249027 cites W1975771588 @default.
• W1994249027 cites W1982012605 @default.
• W1994249027 cites W1985353459 @default.
• W1994249027 cites W1985540529 @default.
• W1994249027 cites W1986240447 @default.
• W1994249027 cites W1987377651 @default.
• W1994249027 cites W1990898399 @default.
• W1994249027 cites W1992319969 @default.
• W1994249027 cites W1995725157 @default.
• W1994249027 cites W2003361133 @default.
• W1994249027 cites W2006296579 @default.
• W1994249027 cites W2007446093 @default.
• W1994249027 cites W2010358621 @default.
• W1994249027 cites W2014231825 @default.
• W1994249027 cites W2016542329 @default.
• W1994249027 cites W2019641834 @default.
• W1994249027 cites W2024596007 @default.
• W1994249027 cites W2029479735 @default.
• W1994249027 cites W2031082470 @default.
• W1994249027 cites W2033241060 @default.
• W1994249027 cites W2036444297 @default.
• W1994249027 cites W2038152815 @default.
• W1994249027 cites W2052188273 @default.
• W1994249027 cites W2061013061 @default.
• W1994249027 cites W2064960805 @default.
• W1994249027 cites W2068615039 @default.
• W1994249027 cites W2068731902 @default.
• W1994249027 cites W2068913774 @default.
• W1994249027 cites W2069535645 @default.
• W1994249027 cites W2077151612 @default.
• W1994249027 cites W2077879155 @default.
• W1994249027 cites W2085694693 @default.
• W1994249027 cites W2091252938 @default.
• W1994249027 cites W2105756791 @default.
• W1994249027 cites W2131019594 @default.
• W1994249027 cites W2131970139 @default.
• W1994249027 cites W2147785018 @default.
• W1994249027 cites W2155832793 @default.
• W1994249027 cites W2158177029 @default.
• W1994249027 cites W2161384920 @default.
• W1994249027 cites W2167373328 @default.
• W1994249027 cites W2167451874 @default.
• W1994249027 cites W2200436717 @default.
• W1994249027 doi "https://doi.org/10.1021/bi00270a029" @default.
• W1994249027 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6830759" @default.
• W1994249027 hasPublicationYear "1983" @default.
• W1994249027 type Work @default.
• W1994249027 sameAs 1994249027 @default.
• W1994249027 citedByCount "16" @default.
• W1994249027 countsByYear W19942490272016 @default.
• W1994249027 crossrefType "journal-article" @default.
• W1994249027 hasAuthorship W1994249027A5047434116 @default.
• W1994249027 hasAuthorship W1994249027A5086815695 @default.
• W1994249027 hasConcept C121332964 @default.
• W1994249027 hasConcept C121745418 @default.
• W1994249027 hasConcept C12554922 @default.
• W1994249027 hasConcept C130316041 @default.
• W1994249027 hasConcept C178790620 @default.
• W1994249027 hasConcept C179998833 @default.
• W1994249027 hasConcept C185592680 @default.
• W1994249027 hasConcept C192468462 @default.
• W1994249027 hasConcept C202033177 @default.
• W1994249027 hasConcept C2776923573 @default.
• W1994249027 hasConcept C2778015030 @default.
• W1994249027 hasConcept C2778918659 @default.
• W1994249027 hasConcept C2778987810 @default.
• W1994249027 hasConcept C2780626841 @default.
• W1994249027 hasConcept C2780827179 @default.
• W1994249027 hasConcept C40875361 @default.
• W1994249027 hasConcept C41625074 @default.
• W1994249027 hasConcept C43617362 @default.
• W1994249027 hasConcept C55493867 @default.
• W1994249027 hasConcept C62520636 @default.
• W1994249027 hasConcept C75473681 @default.
• W1994249027 hasConcept C86803240 @default.
• W1994249027 hasConcept C91881484 @default.
• W1994249027 hasConceptScore W1994249027C121332964 @default.
• W1994249027 hasConceptScore W1994249027C121745418 @default.

• next