Matches in SemOpenAlex for { <https://semopenalex.org/work/W1994249600> ?p ?o ?g. }
- W1994249600 endingPage "28" @default.
- W1994249600 startingPage "13" @default.
- W1994249600 abstract "The corticotropin-releasing hormone (CRH) neurosecretory system in normal rats consists of two major subpopulations of parvicellular neurons in the hypothalamic paraventricular nucleus distinguished by the presence or absence of coexistent vasopressin precursor (pro-AVP)-derived peptides. These neurons project to the external zone of the median eminence, where the two subtypes of axons (CRH+/AVP+ and CRH+/AVP-) were previously found to be approximately equal in number. The present study was undertaken (1) to determine whether the relative numbers of pro-AVP expressing and pro-AVP deficient perikarya in the paraventricular nucleus corresponded to what we previously found for the axons in the median eminence, (2) to map the two cell types throughout the entire paraventricular nucleus to determine whether significant differences existed in their distributions, and (3) to ascertain whether or not the pro-AVP deficient subpopulation expressed pro-AVP after adrenalectomy. Postembedding electron microscopic immunocytochemistry on serial ultrathin sections was used to identify the peptide phenotypes of perikarya in the paraventricular nucleus in normal rats and 7 days after adrenalectomy with and without colchicine treatment. The peptide phenotypes of neuronal perikarya in the paraventricular nucleus were identified by using antibodies to CRH, AVP, neurophysin (NP), the C-terminal glycopeptide of pro-AVP (GP), and oxytocin-associated neurophysin (NPOT). Groups of three serial coronal ultrathin sections were analyzed at 200-μm intervals throughout the entire rostrocaudal extent of the paraventricular nucleus. The sections in each group were stained for CRH, a pro-AVP-derived peptide (AVP, NP or GP), and NPOT, respectively. Parvicellular CRH neurons were defined as CRH-positive cells, approximately 10 μm in diameter, that did not contain detectable NPOT. Pro-AVP expressing cells were defined as staining positively for AVP, GP, or NP and negatively for NPOT. Approximately equal numbers of pro-AVP expressing (“NPAVP+”) and pro-AVP deficient (“NPAVP-”) parvicellular CRH neurons were found within the paraventricular nucleus of colchicine-treated normal rats, and the two subtypes were distributed differently within the paraventricular nucleus. Although the pro-AVP expressing CRH cells stained intensely for NP and GP, staining for AVP was quite variable and difficult to quantify in colchicine-treated normal animals. The CRH+/NPAVP+ neurons were highly concentrated in the dorsal third of the medial parvicellular subdivision of the paraventricular nucleus, in a region roughly midway between the rostral and caudal ends of the paraventricular nucleus, while the CRH+/NPAVP- neurons were more spread out in the medial parvicellular subdivision in both the dorsoventral and the rostrocaudal axes. Adrenalectomy had no effect on the total number of CRH cells or their distribution in colchicine-treated rats. However, more than 90% of the parvicellular, NPOT negative CRH neurons in the paraventricular nucleus stained intensely for AVP, NP, or GP after adrenalectomy and colchicine treatment. The different distributions of the two subpopulations within the paraventricular nucleus in colchicine-treated normal rats suggest possible differences in the nature of their afferent inputs, and the changes induced by adrenalectomy indicate that normal circulating levels of glucocorticoids are sufficient to inhibit expression of pro-AVP in one subpopulation but not the other. However, both subpopulations are regulated by glucocorticoids, as evidenced by the increased staining for AVP in both subpopulations after adrenalectomy. The present results are consistent with our hypothesis of functionally distinct subpopulations of CRH neurons that could modulate the ratio of AVP to CRH in response to specific physiological conditions." @default.
- W1994249600 created "2016-06-24" @default.
- W1994249600 creator A5073068006 @default.
- W1994249600 date "1988-09-01" @default.
- W1994249600 modified "2023-09-26" @default.
- W1994249600 title "Distributions of pro-vasopressin expressing and pro-vasopressin deficient CRH neurons in the paraventricular hypothalamic nucleus of colchicine-treated normal and adrenalectomized rats" @default.
- W1994249600 cites W1509046486 @default.
- W1994249600 cites W1614399163 @default.
- W1994249600 cites W1963542467 @default.
- W1994249600 cites W1964767284 @default.
- W1994249600 cites W1965337556 @default.
- W1994249600 cites W1966682237 @default.
- W1994249600 cites W1972271258 @default.
- W1994249600 cites W1977598948 @default.
- W1994249600 cites W1978837854 @default.
- W1994249600 cites W1980828545 @default.
- W1994249600 cites W1982749261 @default.
- W1994249600 cites W1985705835 @default.
- W1994249600 cites W1987765270 @default.
- W1994249600 cites W1987797235 @default.
- W1994249600 cites W1989992663 @default.
- W1994249600 cites W1993269927 @default.
- W1994249600 cites W2003590296 @default.
- W1994249600 cites W2009064702 @default.
- W1994249600 cites W2009730069 @default.
- W1994249600 cites W2012250657 @default.
- W1994249600 cites W2018400569 @default.
- W1994249600 cites W2019223615 @default.
- W1994249600 cites W2021230596 @default.
- W1994249600 cites W2022174379 @default.
- W1994249600 cites W2022631184 @default.
- W1994249600 cites W2024972430 @default.
- W1994249600 cites W2025547052 @default.
- W1994249600 cites W2026320910 @default.
- W1994249600 cites W2032565714 @default.
- W1994249600 cites W2034251427 @default.
- W1994249600 cites W2036118884 @default.
- W1994249600 cites W2046177214 @default.
- W1994249600 cites W2049779354 @default.
- W1994249600 cites W2051659058 @default.
- W1994249600 cites W2058806365 @default.
- W1994249600 cites W2061136448 @default.
- W1994249600 cites W2061330589 @default.
- W1994249600 cites W2061493394 @default.
- W1994249600 cites W2061939501 @default.
- W1994249600 cites W2067210690 @default.
- W1994249600 cites W2068890204 @default.
- W1994249600 cites W2069419673 @default.
- W1994249600 cites W2074802748 @default.
- W1994249600 cites W2076579939 @default.
- W1994249600 cites W2077163191 @default.
- W1994249600 cites W2080369046 @default.
- W1994249600 cites W2081671508 @default.
- W1994249600 cites W2084576709 @default.
- W1994249600 cites W2084953363 @default.
- W1994249600 cites W2091695321 @default.
- W1994249600 cites W2095036452 @default.
- W1994249600 cites W2114608871 @default.
- W1994249600 cites W2133015759 @default.
- W1994249600 cites W2135472547 @default.
- W1994249600 cites W2145864819 @default.
- W1994249600 cites W2156606373 @default.
- W1994249600 cites W2315654621 @default.
- W1994249600 cites W4242115091 @default.
- W1994249600 cites W4376848280 @default.
- W1994249600 doi "https://doi.org/10.1002/cne.902750103" @default.
- W1994249600 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3262632" @default.
- W1994249600 hasPublicationYear "1988" @default.
- W1994249600 type Work @default.
- W1994249600 sameAs 1994249600 @default.
- W1994249600 citedByCount "71" @default.
- W1994249600 countsByYear W19942496002013 @default.
- W1994249600 countsByYear W19942496002017 @default.
- W1994249600 countsByYear W19942496002020 @default.
- W1994249600 crossrefType "journal-article" @default.
- W1994249600 hasAuthorship W1994249600A5073068006 @default.
- W1994249600 hasConcept C118303440 @default.
- W1994249600 hasConcept C126322002 @default.
- W1994249600 hasConcept C134018914 @default.
- W1994249600 hasConcept C169760540 @default.
- W1994249600 hasConcept C170493617 @default.
- W1994249600 hasConcept C2776176026 @default.
- W1994249600 hasConcept C2776370428 @default.
- W1994249600 hasConcept C2776636253 @default.
- W1994249600 hasConcept C2777003273 @default.
- W1994249600 hasConcept C2777687476 @default.
- W1994249600 hasConcept C2778458438 @default.
- W1994249600 hasConcept C2779067335 @default.
- W1994249600 hasConcept C2780723820 @default.
- W1994249600 hasConcept C35866371 @default.
- W1994249600 hasConcept C6162580 @default.
- W1994249600 hasConcept C71924100 @default.
- W1994249600 hasConcept C86803240 @default.
- W1994249600 hasConceptScore W1994249600C118303440 @default.
- W1994249600 hasConceptScore W1994249600C126322002 @default.
- W1994249600 hasConceptScore W1994249600C134018914 @default.
- W1994249600 hasConceptScore W1994249600C169760540 @default.
- W1994249600 hasConceptScore W1994249600C170493617 @default.