Matches in SemOpenAlex for { <https://semopenalex.org/work/W1994278988> ?p ?o ?g. }
Showing items 1 to 70 of
70
with 100 items per page.
- W1994278988 abstract "MDC1 is a key mediator of DNA damage response in mammals. Here, we show that the N-terminal FHA domain of MDC1 mediates regulated dimerization in response to DNA damage. DNA damage induces phosphorylation of threonine 4 of MDC1 and FHA binds reciprocally to phospho-threonine-4 (pT4) from the other molecule. We determined the crystal structures of the MDC1 FHA domain in the free state and in complex with a pT4 peptide. FHA forms a dimer with pseudo 2-fold symmetry and face-to-face β-sheet contact. The FHA dimer is marginally stable, but is greatly stabilized in vitro and in vivo by the intermolecular binding between FHA and the preceding pT4 peptide. Forms of MDC1 with FHA deletion or mutations compromising dimerization are defective in foci formation at the sites of DNA damage, but could be largely rescued by an artificial dimerization module. Our results support a novel regulation mechanism of MDC1 function through T4 phosphorylation and FHA-mediated dimerization. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr LB-194. doi:10.1158/1538-7445.AM2011-LB-194" @default.
- W1994278988 created "2016-06-24" @default.
- W1994278988 creator A5003581530 @default.
- W1994278988 creator A5009455412 @default.
- W1994278988 creator A5053152816 @default.
- W1994278988 creator A5066312807 @default.
- W1994278988 creator A5067603927 @default.
- W1994278988 date "2011-04-15" @default.
- W1994278988 modified "2023-09-25" @default.
- W1994278988 title "Abstract LB-194: Phosphorylation-dependent dimerization of forkhead-associated domain regulates MDC1 function in response to DNA damage" @default.
- W1994278988 doi "https://doi.org/10.1158/1538-7445.am2011-lb-194" @default.
- W1994278988 hasPublicationYear "2011" @default.
- W1994278988 type Work @default.
- W1994278988 sameAs 1994278988 @default.
- W1994278988 citedByCount "0" @default.
- W1994278988 crossrefType "proceedings-article" @default.
- W1994278988 hasAuthorship W1994278988A5003581530 @default.
- W1994278988 hasAuthorship W1994278988A5009455412 @default.
- W1994278988 hasAuthorship W1994278988A5053152816 @default.
- W1994278988 hasAuthorship W1994278988A5066312807 @default.
- W1994278988 hasAuthorship W1994278988A5067603927 @default.
- W1994278988 hasConcept C11960822 @default.
- W1994278988 hasConcept C134935766 @default.
- W1994278988 hasConcept C143425029 @default.
- W1994278988 hasConcept C153911025 @default.
- W1994278988 hasConcept C178790620 @default.
- W1994278988 hasConcept C185592680 @default.
- W1994278988 hasConcept C2779546866 @default.
- W1994278988 hasConcept C552990157 @default.
- W1994278988 hasConcept C55493867 @default.
- W1994278988 hasConcept C86803240 @default.
- W1994278988 hasConcept C95444343 @default.
- W1994278988 hasConceptScore W1994278988C11960822 @default.
- W1994278988 hasConceptScore W1994278988C134935766 @default.
- W1994278988 hasConceptScore W1994278988C143425029 @default.
- W1994278988 hasConceptScore W1994278988C153911025 @default.
- W1994278988 hasConceptScore W1994278988C178790620 @default.
- W1994278988 hasConceptScore W1994278988C185592680 @default.
- W1994278988 hasConceptScore W1994278988C2779546866 @default.
- W1994278988 hasConceptScore W1994278988C552990157 @default.
- W1994278988 hasConceptScore W1994278988C55493867 @default.
- W1994278988 hasConceptScore W1994278988C86803240 @default.
- W1994278988 hasConceptScore W1994278988C95444343 @default.
- W1994278988 hasLocation W19942789881 @default.
- W1994278988 hasOpenAccess W1994278988 @default.
- W1994278988 hasPrimaryLocation W19942789881 @default.
- W1994278988 hasRelatedWork W1771373428 @default.
- W1994278988 hasRelatedWork W1969400514 @default.
- W1994278988 hasRelatedWork W1988906504 @default.
- W1994278988 hasRelatedWork W1989792012 @default.
- W1994278988 hasRelatedWork W2001822006 @default.
- W1994278988 hasRelatedWork W2003842525 @default.
- W1994278988 hasRelatedWork W2063046876 @default.
- W1994278988 hasRelatedWork W2080964628 @default.
- W1994278988 hasRelatedWork W2096185220 @default.
- W1994278988 hasRelatedWork W2138042601 @default.
- W1994278988 hasRelatedWork W2172127266 @default.
- W1994278988 hasRelatedWork W2182542333 @default.
- W1994278988 hasRelatedWork W2411014700 @default.
- W1994278988 hasRelatedWork W2586089906 @default.
- W1994278988 hasRelatedWork W2895695234 @default.
- W1994278988 hasRelatedWork W2951644440 @default.
- W1994278988 hasRelatedWork W2967469592 @default.
- W1994278988 hasRelatedWork W2978023057 @default.
- W1994278988 hasRelatedWork W3001949391 @default.
- W1994278988 hasRelatedWork W3115309223 @default.
- W1994278988 isParatext "false" @default.
- W1994278988 isRetracted "false" @default.
- W1994278988 magId "1994278988" @default.
- W1994278988 workType "article" @default.