FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1994322556> ?p ?o ?g. }

• W1994322556 abstract "The role of regucalcin, a regulatory protein in intracellular signaling pathway, in cell death was investigated by using the cloned rat hepatoma H4-II-E cells overexpressing regucalcin. The hepatoma cells (wild-type) and stable regucalcin/pCXN2 transfectants were cultured for 72 h in a medium containing 10% fetal bovine serum (FBS) to obtain subconfluent monolayers. After culture for 72 h, cells were further cultured for 24-72 h in a medium containing either vehicle, insulin (10(-8) or 10(-7) M) or insulin-like growth factor-I (IGF-I; 10(-9) or 10(-8) M) in the absence of FBS. The number of wild-type cells was significantly decreased by culture for 24, 48, or 72 h in the presence of insulin (10(-8) or 10(-7) M) or IGF-I (10(-9) or 10(-8) M). Agarose gel electrophoresis showed the presence of low-molecular-weight deoxyribonucleic acid (DNA) fragments of adherent wild-type cells cultured with insulin or IGF-I. The effect of insulin or IGF-I in stimulating cell death and DNA fragmentation in hepatoma cells (wild-type) was significantly prevented in transfectants overexpressing regucalcin. Meanwhile, epinephrine (10(-6) or 10(-5) M) or transforming growth factor-beta1 (10(-13) or 10(-12) M) did not cause cell death of hepatoma cells. Insulin-induced decrease in the number of wild-type cells was significantly prevented by culture with caspase-3 inhibitor (10(-8) M), although the effect of IGF-I was not inhibited. The effect of insulin or IGF-I in inducing the death of hepatoma cells (wild-type) was significantly prevented in the presence of N omega-nitro-L-arginine methylester (NAME), an inhibitor of nitric oxide synthase. Genistein (10(-6) M), an inhibitor of protein tyrosine kinase, or vanadate (10(-5) M), an inhibitor of protein tyrosine phosphatase, caused a significant decrease in the number of hepatoma cells (wild-type). The effect of insulin in inducing the death of wild-type cells was not seen in the presence of genistein or vanadate. The effect of IGF-I on the death of wild-type cells was observed in the presence of genistein or vanadate. The effect of genistein on cell death was significantly prevented in transfectants. Such effect was not seen with vanadate. This study demonstrates that insulin or IGF-I stimulates cell death and apoptosis in the hepatoma cells, and that overexpression of regucalcin has a suppressive effect on cell death induced by insulin or IGF-I that is mediated through different signaling pathway." @default.
• W1994322556 created "2016-06-24" @default.
• W1994322556 creator A5022328074 @default.
• W1994322556 creator A5051437482 @default.
• W1994322556 date "2005-01-01" @default.
• W1994322556 modified "2023-09-23" @default.
• W1994322556 title "Overexpression of regucalcin suppresses cell death and apoptosis in cloned rat hepatoma H4-II-E cells induced by insulin or insulin-like growth factor-I" @default.
• W1994322556 cites W1484536008 @default.
• W1994322556 cites W1964504843 @default.
• W1994322556 cites W1971926903 @default.
• W1994322556 cites W1973708637 @default.
• W1994322556 cites W1977434298 @default.
• W1994322556 cites W1980420000 @default.
• W1994322556 cites W1988605790 @default.
• W1994322556 cites W1990806563 @default.
• W1994322556 cites W1996801850 @default.
• W1994322556 cites W1997286248 @default.
• W1994322556 cites W2007090834 @default.
• W1994322556 cites W2011224377 @default.
• W1994322556 cites W2012525123 @default.
• W1994322556 cites W2023511863 @default.
• W1994322556 cites W2036843530 @default.
• W1994322556 cites W2040998738 @default.
• W1994322556 cites W2043451831 @default.
• W1994322556 cites W2048162805 @default.
• W1994322556 cites W2054914725 @default.
• W1994322556 cites W2056342811 @default.
• W1994322556 cites W2063806193 @default.
• W1994322556 cites W2065834428 @default.
• W1994322556 cites W2069842719 @default.
• W1994322556 cites W2070864575 @default.
• W1994322556 cites W2084398452 @default.
• W1994322556 cites W2130690300 @default.
• W1994322556 cites W2136453191 @default.
• W1994322556 cites W2157548305 @default.
• W1994322556 cites W2313423833 @default.
• W1994322556 cites W2344856292 @default.
• W1994322556 cites W73136315 @default.
• W1994322556 doi "https://doi.org/10.1002/jcb.20485" @default.
• W1994322556 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15880694" @default.
• W1994322556 hasPublicationYear "2005" @default.
• W1994322556 type Work @default.
• W1994322556 sameAs 1994322556 @default.
• W1994322556 citedByCount "11" @default.
• W1994322556 countsByYear W19943225562012 @default.
• W1994322556 countsByYear W19943225562013 @default.
• W1994322556 countsByYear W19943225562016 @default.
• W1994322556 countsByYear W19943225562020 @default.
• W1994322556 crossrefType "journal-article" @default.
• W1994322556 hasAuthorship W1994322556A5022328074 @default.
• W1994322556 hasAuthorship W1994322556A5051437482 @default.
• W1994322556 hasConcept C126322002 @default.
• W1994322556 hasConcept C134018914 @default.
• W1994322556 hasConcept C153911025 @default.
• W1994322556 hasConcept C170493617 @default.
• W1994322556 hasConcept C190283241 @default.
• W1994322556 hasConcept C2775960820 @default.
• W1994322556 hasConcept C2779306644 @default.
• W1994322556 hasConcept C2779404806 @default.
• W1994322556 hasConcept C2780689927 @default.
• W1994322556 hasConcept C31573885 @default.
• W1994322556 hasConcept C43759708 @default.
• W1994322556 hasConcept C54355233 @default.
• W1994322556 hasConcept C55493867 @default.
• W1994322556 hasConcept C71924100 @default.
• W1994322556 hasConcept C81885089 @default.
• W1994322556 hasConcept C86803240 @default.
• W1994322556 hasConceptScore W1994322556C126322002 @default.
• W1994322556 hasConceptScore W1994322556C134018914 @default.
• W1994322556 hasConceptScore W1994322556C153911025 @default.
• W1994322556 hasConceptScore W1994322556C170493617 @default.
• W1994322556 hasConceptScore W1994322556C190283241 @default.
• W1994322556 hasConceptScore W1994322556C2775960820 @default.
• W1994322556 hasConceptScore W1994322556C2779306644 @default.
• W1994322556 hasConceptScore W1994322556C2779404806 @default.
• W1994322556 hasConceptScore W1994322556C2780689927 @default.
• W1994322556 hasConceptScore W1994322556C31573885 @default.
• W1994322556 hasConceptScore W1994322556C43759708 @default.
• W1994322556 hasConceptScore W1994322556C54355233 @default.
• W1994322556 hasConceptScore W1994322556C55493867 @default.
• W1994322556 hasConceptScore W1994322556C71924100 @default.
• W1994322556 hasConceptScore W1994322556C81885089 @default.
• W1994322556 hasConceptScore W1994322556C86803240 @default.
• W1994322556 hasLocation W19943225561 @default.
• W1994322556 hasLocation W19943225562 @default.
• W1994322556 hasOpenAccess W1994322556 @default.
• W1994322556 hasPrimaryLocation W19943225561 @default.
• W1994322556 hasRelatedWork W1975650642 @default.
• W1994322556 hasRelatedWork W2019149333 @default.
• W1994322556 hasRelatedWork W2044988659 @default.
• W1994322556 hasRelatedWork W2051039150 @default.
• W1994322556 hasRelatedWork W2052249995 @default.
• W1994322556 hasRelatedWork W2060694919 @default.
• W1994322556 hasRelatedWork W2089387278 @default.
• W1994322556 hasRelatedWork W2089518257 @default.
• W1994322556 hasRelatedWork W2107322230 @default.
• W1994322556 hasRelatedWork W2430212860 @default.
• W1994322556 isParatext "false" @default.
• W1994322556 isRetracted "false" @default.
• W1994322556 magId "1994322556" @default.

• next