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- W1994361222 abstract "Initial ribulose 1,5-biphosphate carboxylase activity (that present in vivo) and total activity (that which is made available after complete activation by CO2 and Mg2+ were measured in extracts of field-grown soybean (Glycine max cv. Will) leaves collected over a diurnal period. Initial activity increased with increasing irradiance, saturating at 1.2 mmol m−2 s−1, and decreased when irradiance fell below this level. Total activity increased about 3-fold during the first 4 h and remained constant for the rest of the period. Initial activity as a percentage of total activity was about 50% at the beginning and end of the diurnal period and remained near 100% for 6 h in the middle of the day. Leaf photosynthesis and percent activation both increased similarly with increasing irradiance. The lower total activity observed in the morning hours could not be increased by gel-filtration of the extracts in the presence of Mg2+ and CO2 concentrations favoring complete activation. No marked changes were observed in the amount of total leaf protein on a dry weight basis in leaf samples collected throughout the diurnal period. We propose that light has two effects on ribulose 1,5-biphosphate carboxylase activity: (1) light facilitates activation by CO2 and Mg2+, and (2) promotes the conversion of inactive enzyme present after a prolonged dark period to a form which is able to be activated." @default.
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- W1994361222 date "1984-06-01" @default.
- W1994361222 modified "2023-09-26" @default.
- W1994361222 title "Diurnal changes in ribulose 1,5-bisphosphate carboxylase activity and activation state in leaves of field-grown soybeans" @default.
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- W1994361222 doi "https://doi.org/10.1016/0304-4211(84)90184-6" @default.
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