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- W1994367834 abstract "Fiber degradation in the ruminant digestive process is a major activity accomplished by rumen microbes, a process in which the role of fungi is important. Therefore, the present study was conducted to establish the community structure of anaerobic rumen fungi in cattle fed on a high fiber diet using molecular approaches. Total community DNA was extracted, and the ribosomal internal transcribed spacer (ITS) 1 region was amplified, cloned, and sequenced. The resulting nucleotide sequences were used to construct a phylogenetic tree. A total of 52 clones were analyzed, revealing 31 different ITS1 gene phylotypes. Of these, 12 belonged to the genus Orpinomyces (48 % of clones), followed by uncultured Neocallimastigale clones (29 %), Cyllamyces spp. (9 %) and Anaeromyces spp. (8 %). Our results indicate that genus Orpinomyces dominates the rumen fungal community in Indian crossbred Karan Fries cattle." @default.
- W1994367834 created "2016-06-24" @default.
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- W1994367834 date "2013-03-03" @default.
- W1994367834 modified "2023-10-08" @default.
- W1994367834 title "Ribosomal ITS1 sequence-based diversity analysis of anaerobic rumen fungi in cattle fed on high fiber diet" @default.
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- W1994367834 doi "https://doi.org/10.1007/s13213-013-0620-2" @default.
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