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- W1994389546 abstract "The corneoscleral rim of the eye represents a region with unique anatomical properties due to its location between the cornea and sclera and unique functional properties due to the location of epithelial stem cells in the rim structure (limbus). They guarantee the regeneration of the corneal epithelium and prevent the conjunctival epithelium from growing onto the corneal surface. Survival and self-renewal properties of stem cells depend on specific biological and biomechanical properties of their environment. We therefore measured the local mechanical properties of the human corneoscleral rim using a novel nanoindentation device (Bioindenter CSM Instruments, Neuchatel, Switzerland) developed for soft tissues evaluation. Nanoindentation was performed using a spherical indenter of 0,5mm radius, a maximal load ranging between 20 μN to 30 μN and a penetration depth of several μm to 60μm. The hold period at maximum load was 180 seconds. Young's modulus (E) was calculated using a Hertzian fit to the loading data. E of the central cornea was in the range of 19 kPa, while in the scleral region we found 17 kPa and the limbal rim region 10 kPa. Considerable creep relaxation occurred during the hold period at maximum load, which scaled with the elastic modulus of the different structures. For a better correlation of the indentation results to the morphological characteristics of the corneascleral rim, histological sections of the indented areas were performed. Our results reveal unique biomechanical properties of the corneoscleral rim with distinct mechanical properties for the three anatomical regions. Moreover, the corneoscleral rim appears to provide a very soft biomechanical niche environment for the limbal stem cell. These results may have implications to ex-vivo expansion of limbal stem cells and the development of an artificial limbal stem cell construct for tissue engineering." @default.
- W1994389546 created "2016-06-24" @default.
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- W1994389546 date "2014-01-01" @default.
- W1994389546 modified "2023-09-27" @default.
- W1994389546 title "Nanoindentation Derived Mechanical Properties of the Corneascleral Rim of the Human Eye" @default.
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- W1994389546 doi "https://doi.org/10.1016/j.bpj.2013.11.4324" @default.
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