FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1994390521> ?p ?o ?g. }

• W1994390521 endingPage "362" @default.
• W1994390521 startingPage "352" @default.
• W1994390521 abstract "Many bacteria persist within phagocytes, deploying complex sets of tightly regulated virulence factors to manipulate and survive within host cells. So far, no single factor has been identified that is sufficient to allow intracellular persistence of an otherwise non-pathogenic bacterium. Here we report that the two-component KdpD/KdpE sensor kinase/response regulator of the insect and human pathogen Photorhabdus asymbiotica (Pa) is sufficient to allow a harmless laboratory strain of E. coli to resist phagocytic killing and persist within insect hemocytes, ultimately killing the insect. Screening of a cosmid library of Pa in E. coli by injection into the moth Manduca sexta, previously identified three overlapping clones which caused the insect to cease feeding and subsequently die. Transposon mutagenesis revealed a cosmid encoded kdp high affinity potassium pump regulon was responsible for this phenotype. Gentamycin protection assays and confocal microscopy revealed the cosmid clones were persisting inside insect hemocytes far longer than control bacteria. Cloning and expression of PakdpD/kdpE alone into E. coli recapitulated the phenotype. Bioassay results and transcriptional analysis of various E. coli kdp mutants harboring the Pa kdp genes confirmed that Pa KdpD/KdpE was able to induce the E. coli kdp pump structural genes in response to exposure to insect hemocytes but not blood plasma alone. The finding that Pa KdpD/KdpE can facilitate resistance of E. coli to phagocytic killing suggests a central role for potassium in this process, supporting previous work implicating potassium sensing in virulence of other bacteria and also in the normal process of protease killing of engulfed bacteria by neutrophils." @default.
• W1994390521 created "2016-06-24" @default.
• W1994390521 creator A5010954788 @default.
• W1994390521 creator A5015577836 @default.
• W1994390521 creator A5017044270 @default.
• W1994390521 creator A5057248493 @default.
• W1994390521 creator A5058925997 @default.
• W1994390521 creator A5071384282 @default.
• W1994390521 creator A5084478776 @default.
• W1994390521 date "2010-11-01" @default.
• W1994390521 modified "2023-10-18" @default.
• W1994390521 title "The KdpD/KdpE two-component system of Photorhabdus asymbiotica promotes bacterial survival within M. sexta hemocytes" @default.
• W1994390521 cites W1509597125 @default.
• W1994390521 cites W1553379514 @default.
• W1994390521 cites W1567209750 @default.
• W1994390521 cites W1576095742 @default.
• W1994390521 cites W1602731345 @default.
• W1994390521 cites W1608194938 @default.
• W1994390521 cites W1946376449 @default.
• W1994390521 cites W1963638907 @default.
• W1994390521 cites W1964448572 @default.
• W1994390521 cites W1966262091 @default.
• W1994390521 cites W1970398105 @default.
• W1994390521 cites W1972716746 @default.
• W1994390521 cites W1989499793 @default.
• W1994390521 cites W1998024188 @default.
• W1994390521 cites W1999475244 @default.
• W1994390521 cites W2006670481 @default.
• W1994390521 cites W2018040911 @default.
• W1994390521 cites W2023601084 @default.
• W1994390521 cites W2028573316 @default.
• W1994390521 cites W2032229966 @default.
• W1994390521 cites W2033470486 @default.
• W1994390521 cites W2034689410 @default.
• W1994390521 cites W2039092634 @default.
• W1994390521 cites W2039698003 @default.
• W1994390521 cites W2043528136 @default.
• W1994390521 cites W2074001058 @default.
• W1994390521 cites W2074052009 @default.
• W1994390521 cites W2076425670 @default.
• W1994390521 cites W2084814184 @default.
• W1994390521 cites W2086905640 @default.
• W1994390521 cites W2090947027 @default.
• W1994390521 cites W2093769050 @default.
• W1994390521 cites W2099279481 @default.
• W1994390521 cites W2100130339 @default.
• W1994390521 cites W2100693791 @default.
• W1994390521 cites W2104079300 @default.
• W1994390521 cites W2104223886 @default.
• W1994390521 cites W2107580100 @default.
• W1994390521 cites W2117639785 @default.
• W1994390521 cites W2128915430 @default.
• W1994390521 cites W2136000113 @default.
• W1994390521 cites W2143760169 @default.
• W1994390521 cites W2152354835 @default.
• W1994390521 cites W2152370826 @default.
• W1994390521 cites W2167338052 @default.
• W1994390521 cites W290094515 @default.
• W1994390521 doi "https://doi.org/10.1016/j.jip.2010.09.020" @default.
• W1994390521 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20932844" @default.
• W1994390521 hasPublicationYear "2010" @default.
• W1994390521 type Work @default.
• W1994390521 sameAs 1994390521 @default.
• W1994390521 citedByCount "15" @default.
• W1994390521 countsByYear W19943905212013 @default.
• W1994390521 countsByYear W19943905212014 @default.
• W1994390521 countsByYear W19943905212016 @default.
• W1994390521 countsByYear W19943905212017 @default.
• W1994390521 countsByYear W19943905212018 @default.
• W1994390521 countsByYear W19943905212019 @default.
• W1994390521 countsByYear W19943905212023 @default.
• W1994390521 crossrefType "journal-article" @default.
• W1994390521 hasAuthorship W1994390521A5010954788 @default.
• W1994390521 hasAuthorship W1994390521A5015577836 @default.
• W1994390521 hasAuthorship W1994390521A5017044270 @default.
• W1994390521 hasAuthorship W1994390521A5057248493 @default.
• W1994390521 hasAuthorship W1994390521A5058925997 @default.
• W1994390521 hasAuthorship W1994390521A5071384282 @default.
• W1994390521 hasAuthorship W1994390521A5084478776 @default.
• W1994390521 hasConcept C104317684 @default.
• W1994390521 hasConcept C143065580 @default.
• W1994390521 hasConcept C2776074613 @default.
• W1994390521 hasConcept C2777612826 @default.
• W1994390521 hasConcept C2780312687 @default.
• W1994390521 hasConcept C2781196521 @default.
• W1994390521 hasConcept C28693550 @default.
• W1994390521 hasConcept C35101798 @default.
• W1994390521 hasConcept C4918238 @default.
• W1994390521 hasConcept C523546767 @default.
• W1994390521 hasConcept C54355233 @default.
• W1994390521 hasConcept C547475151 @default.
• W1994390521 hasConcept C59822182 @default.
• W1994390521 hasConcept C60987743 @default.
• W1994390521 hasConcept C62361671 @default.
• W1994390521 hasConcept C86803240 @default.
• W1994390521 hasConcept C89423630 @default.
• W1994390521 hasConceptScore W1994390521C104317684 @default.
• W1994390521 hasConceptScore W1994390521C143065580 @default.

• next