Matches in SemOpenAlex for { <https://semopenalex.org/work/W1994401245> ?p ?o ?g. }
Showing items 1 to 81 of
81
with 100 items per page.
- W1994401245 abstract "Ceratoides lanata is the native sand-fixing plant in deserts of America Continent. As an even-green undershrub with extremely strong antifreeze capability, Ceratoides lanata had been considered as the winterfat and introduced into many countries including China. To explore the mechanism of cold resistance and to clone genes directly involving the strong antifreeze capability, a normalized cold-stressed full-length cDNA library was constructed from the cold-treated leaves of Ceratoides lanata by the method of DSN (duplex-specific nuclease)-based normalization and SMART (switching mechanism at 5' end of RNA transcript) full-length cDNA construction. The quality check showed that the titer of un-amplified cDNA library was about 1.4×106 cfu mL−1 and the average size of cDNA inserts was about 1 150 bp with a recombination rate of 100%. The result of sequencing 187 clones selected randomly revealed that the redundancy of the cold-stressed normalized full-length cDNA library is only 0.59%, and the ratio of full length is 46.3%. Among 187 cDNAs, 24 have homology with known genes or proteins, while the other 163 have no identity, suggesting that most cDNAs are new cold-involved genes from Ceratoides lanata. The analytic result showed that the cold-stressed normalized full-length cDNA library of Ceratoides lanata a was established successfully, and this will benefit the further study on the molecular mechanism of strong antifreeze capability and cloning of cold-resistant genes" @default.
- W1994401245 created "2016-06-24" @default.
- W1994401245 creator A5050185265 @default.
- W1994401245 creator A5055444263 @default.
- W1994401245 creator A5060578310 @default.
- W1994401245 creator A5063550422 @default.
- W1994401245 creator A5069214916 @default.
- W1994401245 creator A5086857425 @default.
- W1994401245 date "2010-06-01" @default.
- W1994401245 modified "2023-09-25" @default.
- W1994401245 title "Establishment and Identification of Cold-Stressed Normalized Full-Length cDNA Library of Ceratoides lanata" @default.
- W1994401245 cites W1555425311 @default.
- W1994401245 cites W1980337680 @default.
- W1994401245 cites W1981327703 @default.
- W1994401245 cites W1988737630 @default.
- W1994401245 cites W1998746328 @default.
- W1994401245 cites W2004832656 @default.
- W1994401245 cites W2006762949 @default.
- W1994401245 cites W2028011624 @default.
- W1994401245 cites W2040367407 @default.
- W1994401245 cites W2043512464 @default.
- W1994401245 cites W2044917527 @default.
- W1994401245 cites W2063110554 @default.
- W1994401245 cites W2077169515 @default.
- W1994401245 cites W2089386492 @default.
- W1994401245 cites W2099409927 @default.
- W1994401245 cites W2130008055 @default.
- W1994401245 cites W2131247983 @default.
- W1994401245 cites W2132581303 @default.
- W1994401245 cites W2518262663 @default.
- W1994401245 doi "https://doi.org/10.1109/icbbe.2010.5517719" @default.
- W1994401245 hasPublicationYear "2010" @default.
- W1994401245 type Work @default.
- W1994401245 sameAs 1994401245 @default.
- W1994401245 citedByCount "0" @default.
- W1994401245 crossrefType "proceedings-article" @default.
- W1994401245 hasAuthorship W1994401245A5050185265 @default.
- W1994401245 hasAuthorship W1994401245A5055444263 @default.
- W1994401245 hasAuthorship W1994401245A5060578310 @default.
- W1994401245 hasAuthorship W1994401245A5063550422 @default.
- W1994401245 hasAuthorship W1994401245A5069214916 @default.
- W1994401245 hasAuthorship W1994401245A5086857425 @default.
- W1994401245 hasConcept C104317684 @default.
- W1994401245 hasConcept C187882448 @default.
- W1994401245 hasConcept C189482833 @default.
- W1994401245 hasConcept C54355233 @default.
- W1994401245 hasConcept C59822182 @default.
- W1994401245 hasConcept C86803240 @default.
- W1994401245 hasConceptScore W1994401245C104317684 @default.
- W1994401245 hasConceptScore W1994401245C187882448 @default.
- W1994401245 hasConceptScore W1994401245C189482833 @default.
- W1994401245 hasConceptScore W1994401245C54355233 @default.
- W1994401245 hasConceptScore W1994401245C59822182 @default.
- W1994401245 hasConceptScore W1994401245C86803240 @default.
- W1994401245 hasLocation W19944012451 @default.
- W1994401245 hasOpenAccess W1994401245 @default.
- W1994401245 hasPrimaryLocation W19944012451 @default.
- W1994401245 hasRelatedWork W2027741217 @default.
- W1994401245 hasRelatedWork W2059220783 @default.
- W1994401245 hasRelatedWork W2077689677 @default.
- W1994401245 hasRelatedWork W2122325438 @default.
- W1994401245 hasRelatedWork W2159649207 @default.
- W1994401245 hasRelatedWork W221399146 @default.
- W1994401245 hasRelatedWork W2357494842 @default.
- W1994401245 hasRelatedWork W2372725077 @default.
- W1994401245 hasRelatedWork W2376016810 @default.
- W1994401245 hasRelatedWork W2379572022 @default.
- W1994401245 hasRelatedWork W2381875469 @default.
- W1994401245 hasRelatedWork W2384216705 @default.
- W1994401245 hasRelatedWork W2386475046 @default.
- W1994401245 hasRelatedWork W2387903907 @default.
- W1994401245 hasRelatedWork W2389172935 @default.
- W1994401245 hasRelatedWork W2393964669 @default.
- W1994401245 hasRelatedWork W2577283363 @default.
- W1994401245 hasRelatedWork W3145942239 @default.
- W1994401245 hasRelatedWork W2843950241 @default.
- W1994401245 hasRelatedWork W2851212887 @default.
- W1994401245 isParatext "false" @default.
- W1994401245 isRetracted "false" @default.
- W1994401245 magId "1994401245" @default.
- W1994401245 workType "article" @default.