Matches in SemOpenAlex for { <https://semopenalex.org/work/W1994408300> ?p ?o ?g. }
Showing items 1 to 100 of
100
with 100 items per page.
- W1994408300 endingPage "179" @default.
- W1994408300 startingPage "170" @default.
- W1994408300 abstract "Measurement of DNA damage and repair at the nucleotide level in intact cells has provided compelling evidence for the molecular details of these events as they occur in intact organisms. Furthermore, these measurements give the most accurate picture of the rates of repair in different structural domains of DNA in chromatin. In this report, we describe two methods currently used in our laboratories to map DNA lesions at (or near) nucleotide resolution in yeast cells. The low-resolution method couples damage-specific strand breaks in DNA with indirect end-labeling to measure DNA lesions over a span of 1.5 to 2 kb of DNA sequence. The resolution of this method is limited by the resolution of DNA length measurements on alkaline agarose gels (about +/-20 bp on average). The high-resolution method uses streptavidin magnetic beads and special biotinylated oligonucleotides to facilitate end-labeling of DNA fragments specifically cleaved at damage sites. The latter method maps DNA damage sites at nucleotide resolution over a shorter distance (<500 bp), and is constrained to the length of DNA resolvable on DNA sequencing gels. These methods are used in tandem for answering questions regarding DNA damage and repair in different chromatin domains and states of gene expression." @default.
- W1994408300 created "2016-06-24" @default.
- W1994408300 creator A5024314954 @default.
- W1994408300 creator A5052358177 @default.
- W1994408300 creator A5080261955 @default.
- W1994408300 date "2000-10-01" @default.
- W1994408300 modified "2023-09-29" @default.
- W1994408300 title "Low- and High-Resolution Mapping of DNA Damage at Specific Sites" @default.
- W1994408300 cites W1964590925 @default.
- W1994408300 cites W1965929121 @default.
- W1994408300 cites W1967702447 @default.
- W1994408300 cites W1973170344 @default.
- W1994408300 cites W1986460798 @default.
- W1994408300 cites W1990614202 @default.
- W1994408300 cites W1998025895 @default.
- W1994408300 cites W2003843690 @default.
- W1994408300 cites W2013255015 @default.
- W1994408300 cites W2014689788 @default.
- W1994408300 cites W2015232786 @default.
- W1994408300 cites W2019342009 @default.
- W1994408300 cites W2019457514 @default.
- W1994408300 cites W2029302273 @default.
- W1994408300 cites W2032174390 @default.
- W1994408300 cites W2033533378 @default.
- W1994408300 cites W2042543706 @default.
- W1994408300 cites W2066288618 @default.
- W1994408300 cites W2099332337 @default.
- W1994408300 cites W2102699299 @default.
- W1994408300 cites W2120711292 @default.
- W1994408300 cites W2126190092 @default.
- W1994408300 cites W2131626832 @default.
- W1994408300 cites W2154933591 @default.
- W1994408300 cites W2159923550 @default.
- W1994408300 doi "https://doi.org/10.1006/meth.2000.1058" @default.
- W1994408300 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11020332" @default.
- W1994408300 hasPublicationYear "2000" @default.
- W1994408300 type Work @default.
- W1994408300 sameAs 1994408300 @default.
- W1994408300 citedByCount "43" @default.
- W1994408300 countsByYear W19944083002014 @default.
- W1994408300 countsByYear W19944083002015 @default.
- W1994408300 countsByYear W19944083002016 @default.
- W1994408300 countsByYear W19944083002017 @default.
- W1994408300 countsByYear W19944083002018 @default.
- W1994408300 countsByYear W19944083002020 @default.
- W1994408300 countsByYear W19944083002021 @default.
- W1994408300 countsByYear W19944083002022 @default.
- W1994408300 countsByYear W19944083002023 @default.
- W1994408300 crossrefType "journal-article" @default.
- W1994408300 hasAuthorship W1994408300A5024314954 @default.
- W1994408300 hasAuthorship W1994408300A5052358177 @default.
- W1994408300 hasAuthorship W1994408300A5080261955 @default.
- W1994408300 hasConcept C129312508 @default.
- W1994408300 hasConcept C13575015 @default.
- W1994408300 hasConcept C143425029 @default.
- W1994408300 hasConcept C153911025 @default.
- W1994408300 hasConcept C2776190903 @default.
- W1994408300 hasConcept C2778204606 @default.
- W1994408300 hasConcept C3017666073 @default.
- W1994408300 hasConcept C51679486 @default.
- W1994408300 hasConcept C52182516 @default.
- W1994408300 hasConcept C54355233 @default.
- W1994408300 hasConcept C552990157 @default.
- W1994408300 hasConcept C83640560 @default.
- W1994408300 hasConcept C86803240 @default.
- W1994408300 hasConceptScore W1994408300C129312508 @default.
- W1994408300 hasConceptScore W1994408300C13575015 @default.
- W1994408300 hasConceptScore W1994408300C143425029 @default.
- W1994408300 hasConceptScore W1994408300C153911025 @default.
- W1994408300 hasConceptScore W1994408300C2776190903 @default.
- W1994408300 hasConceptScore W1994408300C2778204606 @default.
- W1994408300 hasConceptScore W1994408300C3017666073 @default.
- W1994408300 hasConceptScore W1994408300C51679486 @default.
- W1994408300 hasConceptScore W1994408300C52182516 @default.
- W1994408300 hasConceptScore W1994408300C54355233 @default.
- W1994408300 hasConceptScore W1994408300C552990157 @default.
- W1994408300 hasConceptScore W1994408300C83640560 @default.
- W1994408300 hasConceptScore W1994408300C86803240 @default.
- W1994408300 hasIssue "2" @default.
- W1994408300 hasLocation W19944083001 @default.
- W1994408300 hasLocation W19944083002 @default.
- W1994408300 hasOpenAccess W1994408300 @default.
- W1994408300 hasPrimaryLocation W19944083001 @default.
- W1994408300 hasRelatedWork W1584758748 @default.
- W1994408300 hasRelatedWork W1965432489 @default.
- W1994408300 hasRelatedWork W2010018187 @default.
- W1994408300 hasRelatedWork W2010348086 @default.
- W1994408300 hasRelatedWork W2014201779 @default.
- W1994408300 hasRelatedWork W2039530285 @default.
- W1994408300 hasRelatedWork W2163295808 @default.
- W1994408300 hasRelatedWork W2418987332 @default.
- W1994408300 hasRelatedWork W2791745050 @default.
- W1994408300 hasRelatedWork W2594895866 @default.
- W1994408300 hasVolume "22" @default.
- W1994408300 isParatext "false" @default.
- W1994408300 isRetracted "false" @default.
- W1994408300 magId "1994408300" @default.
- W1994408300 workType "article" @default.