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• W1994415510 abstract "During culture in vitro of an ascitic mast-cell tumor of the mouse, P-815, three strains have been developed, two of which were obtained by a cloning-technique. One of these pure strains is near diploid, the other tetraploid. All strains maintain their intracellular levels of heparin, histamine, and 5-hydroxytryptamine (5-HT) in culture. The levels of these three substances and the incorporation of S35-sulfate, glucosamine-1-C14, and glucose-U-C14 into heparin have been compared in the cells both in the mouse and in culture. Strains vary in their contents of these compounds and in their capacity to incorporate S35-sulfate and glucosamine-1-C14 into heparin, but in all cases the amounts of heparin, 5-HT, and histamine are higher in culture than when the same strain of cells are grown in the mouse; similarly, the incorporation of S35-sulfate into heparin is higher in culture. The amounts of the amines, especially of 5-HT, are proportional to the amount of heparin. The three substances are found in the same intracellular fraction. By paper chromatography mast-cell heparin has been resolved into three fractions. The concentration of one of these is proportional both to the amount of heparin, as determined by bioassay, and to the levels of amines, especially 5-HT. This component of heparin is not present in bovine heparin. It was also noted that sulfate and glucosamine in heparin turn over at the same rate. The half-life of heparin in the mast-cells is approximately two and one half days." @default.
• W1994415510 created "2016-06-24" @default.
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• W1994415510 date "1960-06-01" @default.
• W1994415510 modified "2023-10-16" @default.
• W1994415510 title "Heparin, 5-hydroxytryptamine, and histamine in neoplastic mast cells" @default.
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• W1994415510 doi "https://doi.org/10.1016/0006-2952(60)90107-6" @default.
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