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• W1994444892 abstract "A series of novel reagents is described for the photobiotinylation of DNA and protein. The reagents consist of a photoactive ligand (photoprobe) tethered to biotin via a polymethylene or azopolymethylene linker. The photoprobes are 4-azidobenzamido, 2-methoxy-6-azido-9-acridinylamino, 2-diazocyclopentadienylcarboxamido, 4-azido-2-nitroanilino and 8-(3-aminopropyloxy)psoralen. The reagents were irradiated with long-wavelength UV light in the presence of DNA or protein and the biotinylation was monitored by blotting to nitrocellulose and staining with avidin—alkaline phosphatase conjugate. The highest DNA photolabelling was obtained with reagents containing the 4-azidobenzamido or psoralen ligands with an amino group in the linker. The 4-azidobenzamido reagent also exhibited the highest protein labelling. Some of these new photobiotinylation reagents are superior to existing ones." @default.
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• W1994444892 date "1991-04-01" @default.
• W1994444892 modified "2023-09-29" @default.
• W1994444892 title "Azidobenzoyl-, azidoacridinyl-, diazocyclopentadienyl-carbonyl- and 8-propyloxypsoralen photobiotinylation reagents. Syntheses and photoreactions with DNA and protein" @default.
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• W1994444892 doi "https://doi.org/10.1016/1010-6030(91)85027-e" @default.
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