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- W1994459939 abstract "A range of new tools in molecular biology are now available to allow the comparative biochemist to explore animal responses to environmental stress at multiple levels. In particular, new techniques of gene discovery, such as cDNA array screening, allow broad assessment of the responses of thousands of genes to a stress. This approach frequently identifies genes (and their associated metabolic functions) that have never before been associated with the stress under study and allows coordinated patterns of gene responses (e.g., by families of genes or by genes encoding multiple proteins in a metabolic pathway or a signal transduction cascade, etc.) to be elucidated. New methods for mRNA (e.g., quantitative PCR) and protein (e.g., peptide antibodies, phospho-specific antibodies) analysis, coupled with major advances in bioinformatics, also simplify the exploration of gene/protein regulation. Techniques and approaches for gene/protein discovery and regulatory analysis are discussed and illustrated with examples drawn from new studies of the responses to anoxia by the marine gastropod, Littorina littorea. D 2004 Elsevier B.V. All rights reserved." @default.
- W1994459939 created "2016-06-24" @default.
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- W1994459939 date "2004-12-01" @default.
- W1994459939 modified "2023-09-26" @default.
- W1994459939 title "Gene regulation in physiological stress" @default.
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- W1994459939 doi "https://doi.org/10.1016/j.ics.2004.09.031" @default.
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