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- W1994469095 abstract "A partially purified enzyme has been prepared from Neurospora crassa which catalyzes the formation of glucosamine phosphate from hexose-6-phosphate and glutamine. The glucosamine phosphate was identified by colour reactions, by dephosphorylation and paper chromatography and by its behaviour towards an acetylating system. Quantitative analysis of amide nitrogen, glutamate, and hexosamine agreed with the following equation: Hexose-6-phosphate + glutamine → glucosamine-6-phosphate + glutamate Crude Neurospora extracts were found to phosphorylate glucosamine in the presence of ATP and, when suitably supplemented, to acetylate glucosamine or glucosamine phosphate. Les auteurs ont préparé et partiellement purifié, à partir de Neurospora crassa, un enzyme qui catalyse la formation de glucosamine phosphate à partir d'hexose-6-phosphate et de glutamine. Le glucosaminephosphate a été identifié par ses réaction colorées, par la déphosphorylation, par la chromatographie sur papier et par son comportement en présence d'un système acétylant. L'analyse quantitative de l'azote amidé, du glutamate et de l'hexosamine est en accord avec l'équation suivante: Hexose-6-phosphate + glutamate → Glucosamine-6-phosphate + glutamate Les extraits bruts de Neurospora phosphorylent la glucosamine en présence d'ATP et écatylent la glucosamine et le glucosaminephosphate, quand on les supplémente convenablement. Ein teilweise gereinigtes Enzym, das die Bildung von Glucosaminphosphat aus Hexose-6-phosphat und Glutamin katalysiert, wurde aus Neurospora crassa dargestellt. Das Glucosaminphosphat wurde durch Farbreaktionen, Desphosphorylierung und Papierchromatographie und durch sein Verhalten gegenüber einem acetylierten System identifiziert. Die quantitative analyse von Amidstickstoff, glutaminsaurem Salz und Hexosamin stimmte mit der folgenden Gleichung überein: Hexose-6-phosphat + Glutamin → Glucosamin-6-phosphat + Glutaminsaures Salz Es wurde gefunden, dass rohe Neurospora-extrakte Glucosamin in Gegenwart von ATP phosphorylieren und, wenn sie geeignet ergänzt werden, Glucosamin oder Glucosaminphosphat acetylieren." @default.
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- W1994469095 date "1953-10-01" @default.
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- W1994469095 title "The biosynthesis of glucosamine" @default.
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- W1994469095 doi "https://doi.org/10.1016/0006-3002(53)90119-x" @default.
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