Matches in SemOpenAlex for { <https://semopenalex.org/work/W1994469123> ?p ?o ?g. }
Showing items 1 to 98 of
98
with 100 items per page.
- W1994469123 endingPage "1964" @default.
- W1994469123 startingPage "1961" @default.
- W1994469123 abstract "ABSTRACT We have isolated a number of vanB -containing Enterococcus faecium isolates on bile esculin screening agar containing 6 mg/liter vancomycin, which on subsequent susceptibility testing using Etest have repeatedly demonstrated vancomycin MICs of ≤4 mg/liter. To investigate this genotype-phenotype incongruence of “low-MIC vancomycin-resistant enterococci” (LM-VRE), we examined the molecular characteristics of these isolates, including the presence of the vanB operon, using PCR amplification and DNA sequencing. All LM-VRE isolates contained vanB associated with the transposon Tn 1549 and were polyclonal. Sequencing of the vanB ligase gene showed no differences from the prototype vanB2 . In addition, we examined supplemented media to improve phenotypic detection of these isolates. Etest detection of LM-VRE improved when Mueller-Hinton agar (MHA) and brain heart infusion agar (BHIA) were supplemented with 10 g/liter oxgall (MHA-Oxg and BHIA-Oxg, respectively). We assessed the sensitivity and specificity of these media to detect vancomycin resistance using vancomycin-resistant vanB -containing E. faecium ( n = 11), vancomycin-susceptible ( van negative) E. faecium ( n = 11), vancomycin-susceptible ( van negative) E. faecalis ( n = 11), and our LM-VRE ( n = 23) isolates. After 48 h of incubation, both MHA-Oxg and BHIA-Oxg were 100% (34/34) sensitive and 100% (22/22) specific in the identification of vancomycin resistance. These findings suggest that supplementation of MHA or BHIA with 10 g/liter oxgall should be considered in laboratories where VRE detection protocols rely primarily on strain phenotype rather than early vanB gene detection by PCR." @default.
- W1994469123 created "2016-06-24" @default.
- W1994469123 creator A5010982342 @default.
- W1994469123 creator A5016230572 @default.
- W1994469123 creator A5028208362 @default.
- W1994469123 creator A5037265480 @default.
- W1994469123 creator A5040528325 @default.
- W1994469123 creator A5074463845 @default.
- W1994469123 creator A5085741718 @default.
- W1994469123 date "2008-06-01" @default.
- W1994469123 modified "2023-09-26" @default.
- W1994469123 title "Improved Detection of <i>vanB2</i> -Containing <i>Enterococcus faecium</i> with Vancomycin Susceptibility by Etest Using Oxgall Supplementation" @default.
- W1994469123 cites W1961220478 @default.
- W1994469123 cites W1963989906 @default.
- W1994469123 cites W2012278332 @default.
- W1994469123 cites W2021215840 @default.
- W1994469123 cites W2031114425 @default.
- W1994469123 cites W2046792208 @default.
- W1994469123 cites W2075368746 @default.
- W1994469123 cites W2077994463 @default.
- W1994469123 cites W2092810704 @default.
- W1994469123 cites W2123328230 @default.
- W1994469123 cites W2141883139 @default.
- W1994469123 cites W2144044424 @default.
- W1994469123 cites W2146311752 @default.
- W1994469123 cites W2169656535 @default.
- W1994469123 cites W2334666826 @default.
- W1994469123 cites W4210969014 @default.
- W1994469123 doi "https://doi.org/10.1128/jcm.01778-07" @default.
- W1994469123 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2446868" @default.
- W1994469123 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18434564" @default.
- W1994469123 hasPublicationYear "2008" @default.
- W1994469123 type Work @default.
- W1994469123 sameAs 1994469123 @default.
- W1994469123 citedByCount "19" @default.
- W1994469123 countsByYear W19944691232012 @default.
- W1994469123 countsByYear W19944691232014 @default.
- W1994469123 countsByYear W19944691232015 @default.
- W1994469123 countsByYear W19944691232016 @default.
- W1994469123 countsByYear W19944691232018 @default.
- W1994469123 countsByYear W19944691232019 @default.
- W1994469123 countsByYear W19944691232022 @default.
- W1994469123 countsByYear W19944691232023 @default.
- W1994469123 crossrefType "journal-article" @default.
- W1994469123 hasAuthorship W1994469123A5010982342 @default.
- W1994469123 hasAuthorship W1994469123A5016230572 @default.
- W1994469123 hasAuthorship W1994469123A5028208362 @default.
- W1994469123 hasAuthorship W1994469123A5037265480 @default.
- W1994469123 hasAuthorship W1994469123A5040528325 @default.
- W1994469123 hasAuthorship W1994469123A5074463845 @default.
- W1994469123 hasAuthorship W1994469123A5085741718 @default.
- W1994469123 hasBestOaLocation W19944691231 @default.
- W1994469123 hasConcept C2777677444 @default.
- W1994469123 hasConcept C2778660310 @default.
- W1994469123 hasConcept C2778980435 @default.
- W1994469123 hasConcept C2779489039 @default.
- W1994469123 hasConcept C2780585763 @default.
- W1994469123 hasConcept C501593827 @default.
- W1994469123 hasConcept C523546767 @default.
- W1994469123 hasConcept C54355233 @default.
- W1994469123 hasConcept C81396022 @default.
- W1994469123 hasConcept C86803240 @default.
- W1994469123 hasConcept C89423630 @default.
- W1994469123 hasConceptScore W1994469123C2777677444 @default.
- W1994469123 hasConceptScore W1994469123C2778660310 @default.
- W1994469123 hasConceptScore W1994469123C2778980435 @default.
- W1994469123 hasConceptScore W1994469123C2779489039 @default.
- W1994469123 hasConceptScore W1994469123C2780585763 @default.
- W1994469123 hasConceptScore W1994469123C501593827 @default.
- W1994469123 hasConceptScore W1994469123C523546767 @default.
- W1994469123 hasConceptScore W1994469123C54355233 @default.
- W1994469123 hasConceptScore W1994469123C81396022 @default.
- W1994469123 hasConceptScore W1994469123C86803240 @default.
- W1994469123 hasConceptScore W1994469123C89423630 @default.
- W1994469123 hasIssue "6" @default.
- W1994469123 hasLocation W19944691231 @default.
- W1994469123 hasLocation W19944691232 @default.
- W1994469123 hasLocation W19944691233 @default.
- W1994469123 hasLocation W19944691234 @default.
- W1994469123 hasOpenAccess W1994469123 @default.
- W1994469123 hasPrimaryLocation W19944691231 @default.
- W1994469123 hasRelatedWork W1994469123 @default.
- W1994469123 hasRelatedWork W2017814274 @default.
- W1994469123 hasRelatedWork W2062867631 @default.
- W1994469123 hasRelatedWork W2086351130 @default.
- W1994469123 hasRelatedWork W2097177512 @default.
- W1994469123 hasRelatedWork W2147613540 @default.
- W1994469123 hasRelatedWork W2164576845 @default.
- W1994469123 hasRelatedWork W2756630929 @default.
- W1994469123 hasRelatedWork W4309508583 @default.
- W1994469123 hasRelatedWork W4361296585 @default.
- W1994469123 hasVolume "46" @default.
- W1994469123 isParatext "false" @default.
- W1994469123 isRetracted "false" @default.
- W1994469123 magId "1994469123" @default.
- W1994469123 workType "article" @default.