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- W1994495352 abstract "Mesophyll cell protoplasts were isolated from tobacco leaves systemically infected by potato virus X (PVX), and over 4000 plants were regenerated from them. Of these, 92.5% were PVX infected, and a broad range of variant virus strains was found to occur among the different protoplast-derived plants. Of the 7.5% of the plants which were not infected, all were susceptible upon reinoculation although one plant required three inoculations with a PVX preparation at 2 mg/ml before systemic infection was established. Uninfected cells from a systemically infected leaf displayed no selective cultural advantage over PVX-infected cells when plated and induced to form colonies. However, when protoplasts were isolated from PVX and potato virus Y (PVY) doubly infected leaves, a pronounced selective advantage was observed for protoplasts which were not doubly infected. Of plants regenerated from protoplasts of PVX-PVY-infected leaves, over 95% were free from viral infection." @default.
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- W1994495352 date "1975-08-01" @default.
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- W1994495352 title "Regeneration of plants from protoplasts of potato virus X-infected tobacco leaves" @default.
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- W1994495352 doi "https://doi.org/10.1016/0042-6822(75)90221-4" @default.
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