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- W1994500979 abstract "The CHO cell temperature-sensitive mutant ldlF exhibits two defects in membrane traffic at the nonpermissive temperature (39.5 degrees C): rapid degradation of LDL receptors, possibly caused by endocytic missorting, and disruption of ER-through-Golgi transport. Here, we show that at 39.5 degrees C, the Golgi in ldlF cells dissociated into vesicles and tubules. This dissociation was inhibited by AlF4-, suggesting trimeric G proteins are involved in the dissociation mechanism. This resembled the effects of brefeldin A on wild-type cells. We isolated a hamster cDNA that specifically corrected the ts defects of ldlF cells, but not those of other similar ts mutants (ldlE, ldlG, ldlH, and End4). Its predicted protein sequence is conserved in humans, rice, Arabidopsis, and Caenorhabditis elegans, and is virtually identical to that of bovine epsilon-COP, a component of the coatomer complex implicated in membrane transport. This provides the first genetic evidence that coatomers in animal cells can play a role both in maintaining Golgi structure and in mediating ER-through-Golgi transport, and can influence normal endocytic recycling of LDL receptors. Thus, along with biochemical and yeast genetics methods, mammalian somatic cell mutants can provide powerful tools for the elucidation of the mechanisms underlying intracellular membrane traffic." @default.
- W1994500979 created "2016-06-24" @default.
- W1994500979 creator A5028038671 @default.
- W1994500979 creator A5074035300 @default.
- W1994500979 creator A5083131568 @default.
- W1994500979 date "1994-06-15" @default.
- W1994500979 modified "2023-10-15" @default.
- W1994500979 title "Disruptions in Golgi structure and membrane traffic in a conditional lethal mammalian cell mutant are corrected by epsilon-COP." @default.
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- W1994500979 doi "https://doi.org/10.1083/jcb.125.6.1213" @default.
- W1994500979 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2290926" @default.
- W1994500979 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8207054" @default.
- W1994500979 hasPublicationYear "1994" @default.