Matches in SemOpenAlex for { <https://semopenalex.org/work/W1994516257> ?p ?o ?g. }
- W1994516257 endingPage "1601" @default.
- W1994516257 startingPage "1595" @default.
- W1994516257 abstract "The 6×-Histidine tag which is commonly used for purification of recombinant proteins was converted to a catalytic redox-active center by incorporation of Co(2+). Two examples of the biological activity of this engineered protein-derived cofactor are presented. After inactivation of the natural diheme cofactor of MauG, it was shown that the Co(2+)-loaded 6×His-tag could substitute for the hemes in the H2O2-driven catalysis of tryptophan tryptophylquinone biosynthesis. To further demonstrate that the Co(2+)-loaded 6×His-tag could mediate long range electron transfer, it was shown that addition of H2O2 to the Co(2+)-loaded 6×His-tagged Cu(1+) amicyanin oxidizes the copper site which is 20Å away. These results provide proof of principle for this simple method by which to introduce a catalytic redox-active site into proteins for potential applications in research and biotechnology." @default.
- W1994516257 created "2016-06-24" @default.
- W1994516257 creator A5038151729 @default.
- W1994516257 creator A5056578032 @default.
- W1994516257 creator A5067994743 @default.
- W1994516257 creator A5087036832 @default.
- W1994516257 date "2014-10-01" @default.
- W1994516257 modified "2023-10-16" @default.
- W1994516257 title "A simple method to engineer a protein-derived redox cofactor for catalysis" @default.
- W1994516257 cites W1516495306 @default.
- W1994516257 cites W1516577124 @default.
- W1994516257 cites W1589880964 @default.
- W1994516257 cites W1675570769 @default.
- W1994516257 cites W1691695228 @default.
- W1994516257 cites W1810520313 @default.
- W1994516257 cites W1964607751 @default.
- W1994516257 cites W1970220520 @default.
- W1994516257 cites W1984649984 @default.
- W1994516257 cites W1990738622 @default.
- W1994516257 cites W1991617851 @default.
- W1994516257 cites W1999809525 @default.
- W1994516257 cites W2015304045 @default.
- W1994516257 cites W2018873978 @default.
- W1994516257 cites W2023023571 @default.
- W1994516257 cites W2027140236 @default.
- W1994516257 cites W2028853592 @default.
- W1994516257 cites W2031358174 @default.
- W1994516257 cites W2031904472 @default.
- W1994516257 cites W2034971743 @default.
- W1994516257 cites W2040111466 @default.
- W1994516257 cites W2045446729 @default.
- W1994516257 cites W2045461231 @default.
- W1994516257 cites W2056268314 @default.
- W1994516257 cites W2057453457 @default.
- W1994516257 cites W2059803436 @default.
- W1994516257 cites W2063337814 @default.
- W1994516257 cites W2064736199 @default.
- W1994516257 cites W2079244126 @default.
- W1994516257 cites W2081246638 @default.
- W1994516257 cites W2084787090 @default.
- W1994516257 cites W2091970958 @default.
- W1994516257 cites W2091992600 @default.
- W1994516257 cites W2098451745 @default.
- W1994516257 cites W2107244464 @default.
- W1994516257 cites W2111876226 @default.
- W1994516257 cites W2117880679 @default.
- W1994516257 cites W2127456273 @default.
- W1994516257 cites W2156448192 @default.
- W1994516257 cites W2223129330 @default.
- W1994516257 cites W267757020 @default.
- W1994516257 cites W87054285 @default.
- W1994516257 cites W2472760289 @default.
- W1994516257 doi "https://doi.org/10.1016/j.bbabio.2014.05.354" @default.
- W1994516257 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4285715" @default.
- W1994516257 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24858537" @default.
- W1994516257 hasPublicationYear "2014" @default.
- W1994516257 type Work @default.
- W1994516257 sameAs 1994516257 @default.
- W1994516257 citedByCount "2" @default.
- W1994516257 countsByYear W19945162572018 @default.
- W1994516257 countsByYear W19945162572022 @default.
- W1994516257 crossrefType "journal-article" @default.
- W1994516257 hasAuthorship W1994516257A5038151729 @default.
- W1994516257 hasAuthorship W1994516257A5056578032 @default.
- W1994516257 hasAuthorship W1994516257A5067994743 @default.
- W1994516257 hasAuthorship W1994516257A5087036832 @default.
- W1994516257 hasBestOaLocation W19945162571 @default.
- W1994516257 hasConcept C123669783 @default.
- W1994516257 hasConcept C147816474 @default.
- W1994516257 hasConcept C161790260 @default.
- W1994516257 hasConcept C178790620 @default.
- W1994516257 hasConcept C181199279 @default.
- W1994516257 hasConcept C181312439 @default.
- W1994516257 hasConcept C185592680 @default.
- W1994516257 hasConcept C197957613 @default.
- W1994516257 hasConcept C21951064 @default.
- W1994516257 hasConcept C2776706248 @default.
- W1994516257 hasConcept C2778460671 @default.
- W1994516257 hasConcept C41183919 @default.
- W1994516257 hasConcept C515207424 @default.
- W1994516257 hasConcept C544778455 @default.
- W1994516257 hasConcept C55493867 @default.
- W1994516257 hasConcept C55904794 @default.
- W1994516257 hasConcept C75473681 @default.
- W1994516257 hasConceptScore W1994516257C123669783 @default.
- W1994516257 hasConceptScore W1994516257C147816474 @default.
- W1994516257 hasConceptScore W1994516257C161790260 @default.
- W1994516257 hasConceptScore W1994516257C178790620 @default.
- W1994516257 hasConceptScore W1994516257C181199279 @default.
- W1994516257 hasConceptScore W1994516257C181312439 @default.
- W1994516257 hasConceptScore W1994516257C185592680 @default.
- W1994516257 hasConceptScore W1994516257C197957613 @default.
- W1994516257 hasConceptScore W1994516257C21951064 @default.
- W1994516257 hasConceptScore W1994516257C2776706248 @default.
- W1994516257 hasConceptScore W1994516257C2778460671 @default.
- W1994516257 hasConceptScore W1994516257C41183919 @default.
- W1994516257 hasConceptScore W1994516257C515207424 @default.
- W1994516257 hasConceptScore W1994516257C544778455 @default.