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- W1994546013 abstract "The localization of histamine formed by human platelets in response to agonists was evaluated. 87 ± 5% of the histamine in a suspension of platelets exposed to phorbol-12-myristate-13-acetate (PMA) was associated with the platelet pellet. Incubation of saponin-permeabilized platelets with the intracellular histamine antagonist, N,N-diethyl-2-[4-(phenylmethyl)phenoxy]ethanamine·HCl (DPPE), released 75 ± 3.9% of the histamine into the supernatant. Under conditions where 90% of platelet serotonin was secreted into the supernatant, the majority (80%) of platelet histamine remained associated with the pellet. The results suggest that histamine synthesized in response to agonists is largely cytoplasmic." @default.
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- W1994546013 date "1989-10-01" @default.
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- W1994546013 title "Histamine formed in stimulated human platelets is cytoplasmic" @default.
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- W1994546013 doi "https://doi.org/10.1016/0006-291x(89)91697-5" @default.
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