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• W1994568157 abstract "Malaria parasite cloning is traditionally carried out mainly by using the limiting dilution method, which is laborious, imprecise, and unable to distinguish multiply-infected RBCs. In this study, we used a parasite engineered to express green fluorescent protein (GFP) to evaluate a single-cell sorting method for rapidly cloning Plasmodium falciparum. By dividing a two-dimensional scattergram from a cell sorter into 17 gates, we determined the parameters for isolating singly-infected erythrocytes and sorted them into individual cultures. Pre-gating of the engineered parasites for GFP allowed the isolation of almost 100% GFP-positive clones. Compared with the limiting dilution method, the number of parasite clones obtained by single-cell sorting was much higher. Molecular analyses showed that parasite isolates obtained by single-cell sorting were highly homogenous. This highly efficient single-cell sorting method should prove very useful for cloning both P. falciparum laboratory populations from genetic manipulation experiments and clinical samples." @default.
• W1994568157 created "2016-06-24" @default.
• W1994568157 creator A5005484398 @default.
• W1994568157 creator A5044817441 @default.
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• W1994568157 date "2010-10-01" @default.
• W1994568157 modified "2023-09-23" @default.
• W1994568157 title "Cloning of Plasmodium falciparum by single-cell sorting" @default.
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• W1994568157 doi "https://doi.org/10.1016/j.exppara.2010.04.022" @default.
• W1994568157 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2914851" @default.
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