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• W1994572714 abstract "Leptin, an appetite-regulating hormone/cytokine, circulates both free and bound to soluble leptin receptors (s-leptin R). An electrochemiluminescence (ECL) assay for the quantitative measurement of murine s-leptin R was developed. The absence of s-leptin R immunoreactivity in the serum ofdbpas/dbpas mice demonstrated the specificity of the assay, which detected s-leptin R both in the free form and complexed with leptin. The distribution of free vs bound leptin and the regulation of s-leptin R were evaluated in mice following administration of the pro-inflammatory stimuli endotoxin (100 μg/mouse) and turpentine (100 μl/mouse). Both endotoxin and turpentine significantly increased serum leptin and s-leptin R levels compared to control mice. The distribution of free vs bound leptin was not altered by administration of endotoxin or turpentine. In fact, approximately 50% of total leptin was present in the free form in either control, endotoxin- or turpentine-injected mice. On the contrary, during the hyperleptinemia of pregnancy, only 10% of total leptin was present in the free form. We conclude that inflammation leads to the increase of both bound and free leptin. Therefore, the total amount of bioactive leptin is increased during acute inflammation, suggesting that leptin participates in the host response to inflammation." @default.
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• W1994572714 date "2001-04-01" @default.
• W1994572714 modified "2023-10-15" @default.
• W1994572714 title "REGULATION OF FREE AND BOUND LEPTIN AND SOLUBLE LEPTIN RECEPTORS DURING INFLAMMATION IN MICE" @default.
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• W1994572714 doi "https://doi.org/10.1006/cyto.2001.0859" @default.
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