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• W1994662078 abstract "Abstract Malondialdehyde (MDA) is very often taken as an index of lipid peroxidation but its measurement by colorimetric methods has been heavily questioned particularly in the case of cultured cells. In the present work, we have thus adapted a gas chromatographic method coupled with nitrogen–phosphorus detection to measure in various cell types (PC12, fibroblasts) the formation of MDA in the cells as well as in the extracellular medium. When measuring MDA formation in cells treated by hydrogen peroxide/ferrous iron, we observed that the addition of increasing concentrations of H2O2/Fe2+ (0.1–1 mm) led to a linear formation of MDA in the cells and in the culture medium (fibroblasts) or in the culture medium only (PC12 cells). In contrast, cumene hydroperoxyde (CHP) failed to induce MDA formation and release in both cell types. In conclusion, our method using hydrazinobenzothiazole (HBT) derivatisation is simple and reliable and sensitive enough to evaluate MDA production at the cellular level and to investigate the role of lipid peroxidation in cell degeneration." @default.
• W1994662078 created "2016-06-24" @default.
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• W1994662078 date "2004-12-28" @default.
• W1994662078 modified "2023-09-26" @default.
• W1994662078 title "Gas Chromatographic Analysis of Malondialdehyde as an Index of Lipid Peroxidation: Validation and Application in Cultured Cells" @default.
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• W1994662078 doi "https://doi.org/10.1081/al-120035903" @default.
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