Matches in SemOpenAlex for { <https://semopenalex.org/work/W1994687513> ?p ?o ?g. }
Showing items 1 to 89 of
89
with 100 items per page.
- W1994687513 endingPage "240" @default.
- W1994687513 startingPage "237" @default.
- W1994687513 abstract "The Ca(2+)-dependent protein phosphatase activity of crude rat brain extracts measured in the presence of okadaic acid, exhibits the characteristic properties of the calmodulin-stimulated protein phosphatase, calcineurin. It is stimulated more than 200-fold by Ca2+ and inhibited by the calmodulin-binding peptide, M13, and by the immunosuppressive drug, FK506. It is insensitive to rapamycin at concentrations up to 1 microM. Its specific activity, based on calcineurin concentration determined by quantitative analysis of Western blots exposed to anti-bovine brain IgG, is ten to twenty times that of purified rat brain calcineurin assayed under similar conditions. Unlike the purified enzyme it is rapidly and irreversibly inactivated in a time-, temperature-, and Ca2+/calmodulin-dependent fashion without evidence of extensive proteolytic degradation. The enzyme is converted to a state which does not lose activity by removal of low molecular weight material by gel filtration. Reconstitution of a labile enzyme is achieved by the addition of the low molecular weight-containing fraction eluted from the gel filtration column. These observations indicate that calcineurin in crude brain extracts is under the control of Ca2+/calmodulin-dependent positive and negative regulatory mechanisms which involve unidentified endogenous factor(s)." @default.
- W1994687513 created "2016-06-24" @default.
- W1994687513 creator A5011099524 @default.
- W1994687513 creator A5019636157 @default.
- W1994687513 creator A5049983845 @default.
- W1994687513 creator A5056024107 @default.
- W1994687513 date "1995-10-30" @default.
- W1994687513 modified "2023-10-17" @default.
- W1994687513 title "Factors responsible for the Ca<sup>2+</sup>‐dependent inactivation of calcineurin in brain" @default.
- W1994687513 cites W1005980004 @default.
- W1994687513 cites W1577039421 @default.
- W1994687513 cites W1775749144 @default.
- W1994687513 cites W1967603109 @default.
- W1994687513 cites W1980660237 @default.
- W1994687513 cites W1984754431 @default.
- W1994687513 cites W1988832534 @default.
- W1994687513 cites W1997883722 @default.
- W1994687513 cites W2035509838 @default.
- W1994687513 cites W2091538386 @default.
- W1994687513 cites W2808792907 @default.
- W1994687513 doi "https://doi.org/10.1016/0014-5793(95)01095-v" @default.
- W1994687513 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7589543" @default.
- W1994687513 hasPublicationYear "1995" @default.
- W1994687513 type Work @default.
- W1994687513 sameAs 1994687513 @default.
- W1994687513 citedByCount "37" @default.
- W1994687513 countsByYear W19946875132012 @default.
- W1994687513 countsByYear W19946875132013 @default.
- W1994687513 countsByYear W19946875132017 @default.
- W1994687513 countsByYear W19946875132018 @default.
- W1994687513 countsByYear W19946875132021 @default.
- W1994687513 crossrefType "journal-article" @default.
- W1994687513 hasAuthorship W1994687513A5011099524 @default.
- W1994687513 hasAuthorship W1994687513A5019636157 @default.
- W1994687513 hasAuthorship W1994687513A5049983845 @default.
- W1994687513 hasAuthorship W1994687513A5056024107 @default.
- W1994687513 hasBestOaLocation W19946875131 @default.
- W1994687513 hasConcept C126322002 @default.
- W1994687513 hasConcept C128057223 @default.
- W1994687513 hasConcept C178666793 @default.
- W1994687513 hasConcept C178790620 @default.
- W1994687513 hasConcept C181199279 @default.
- W1994687513 hasConcept C185592680 @default.
- W1994687513 hasConcept C193363816 @default.
- W1994687513 hasConcept C2776953658 @default.
- W1994687513 hasConcept C2777658833 @default.
- W1994687513 hasConcept C28212737 @default.
- W1994687513 hasConcept C2911091166 @default.
- W1994687513 hasConcept C29688787 @default.
- W1994687513 hasConcept C519063684 @default.
- W1994687513 hasConcept C55493867 @default.
- W1994687513 hasConcept C71924100 @default.
- W1994687513 hasConceptScore W1994687513C126322002 @default.
- W1994687513 hasConceptScore W1994687513C128057223 @default.
- W1994687513 hasConceptScore W1994687513C178666793 @default.
- W1994687513 hasConceptScore W1994687513C178790620 @default.
- W1994687513 hasConceptScore W1994687513C181199279 @default.
- W1994687513 hasConceptScore W1994687513C185592680 @default.
- W1994687513 hasConceptScore W1994687513C193363816 @default.
- W1994687513 hasConceptScore W1994687513C2776953658 @default.
- W1994687513 hasConceptScore W1994687513C2777658833 @default.
- W1994687513 hasConceptScore W1994687513C28212737 @default.
- W1994687513 hasConceptScore W1994687513C2911091166 @default.
- W1994687513 hasConceptScore W1994687513C29688787 @default.
- W1994687513 hasConceptScore W1994687513C519063684 @default.
- W1994687513 hasConceptScore W1994687513C55493867 @default.
- W1994687513 hasConceptScore W1994687513C71924100 @default.
- W1994687513 hasIssue "2" @default.
- W1994687513 hasLocation W19946875131 @default.
- W1994687513 hasLocation W19946875132 @default.
- W1994687513 hasOpenAccess W1994687513 @default.
- W1994687513 hasPrimaryLocation W19946875131 @default.
- W1994687513 hasRelatedWork W131335327 @default.
- W1994687513 hasRelatedWork W1840326104 @default.
- W1994687513 hasRelatedWork W1999919687 @default.
- W1994687513 hasRelatedWork W2024589487 @default.
- W1994687513 hasRelatedWork W2037859627 @default.
- W1994687513 hasRelatedWork W2073682105 @default.
- W1994687513 hasRelatedWork W2086141071 @default.
- W1994687513 hasRelatedWork W2265438201 @default.
- W1994687513 hasRelatedWork W2350267216 @default.
- W1994687513 hasRelatedWork W2395411246 @default.
- W1994687513 hasVolume "374" @default.
- W1994687513 isParatext "false" @default.
- W1994687513 isRetracted "false" @default.
- W1994687513 magId "1994687513" @default.
- W1994687513 workType "article" @default.