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- W1994696054 abstract "The Staudinger ligation between an azido-protein and a phosphinothioester-derivatized surface is demonstrated to be an effective means for the site-specific, covalent immobilization of a protein. Immobilization yields of >50% are obtained in <1 min, and immobilized proteins have >80% of their expected activity. No other method enables more rapid immobilization or a higher yield of active protein. Because azido-peptides and azido-proteins are readily attainable by synthesis, biosynthesis, or semisynthesis, the Staudinger ligation could be of unsurpassed utility in creating microarrays of functional peptides and proteins." @default.
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- W1994696054 date "2003-09-04" @default.
- W1994696054 modified "2023-10-16" @default.
- W1994696054 title "Site-Specific Protein Immobilization by Staudinger Ligation" @default.
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- W1994696054 doi "https://doi.org/10.1021/ja036712h" @default.
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