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- W1994770692 abstract "A convenient acidimetric assay for phospholipase A using egg yolk suspension as substrate has been developed. The substrate mixture consists of 1 part egg yolk, 1 part 8.1 mm sodium deoxycholate, and 1 part 18 mm calcium chloride. Phospholipase A activity is measured by following the initial rate of pH change, which is linear between pH 8.0 and 7.75 and is proportional to enzyme concentration over a wide range. The assay is highly reproducible, with a coefficient of variation of 3%, and as sensitive as most established assays for phospholipase A. The assay uses inexpensive and easily available substrate and is simple to perform. It is particularly useful for monitoring phospholipase A activity in chromatography fractions." @default.
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- W1994770692 date "1988-05-01" @default.
- W1994770692 modified "2023-10-16" @default.
- W1994770692 title "Acidimetric assay for phospholipase A using egg yolk suspension as substrate" @default.
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- W1994770692 doi "https://doi.org/10.1016/0003-2697(88)90632-x" @default.
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