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• W1994771235 abstract "Abstract Based on the observation that T7 RNAP binds reversibly to the polyaromatic sulphonated triazine dye, cibacron blue, we have developed a rapid purification procedure for T7 RNAP. It employs chromatography of the ammonium sulfate fraction through a blue sepharose column, which has the dye coupled to the solid sepharose support. The enzyme can be eluted by 2M NaCl or 1M NaCl together with 1 mM UTP. These methods are compared with another purification procedure using ion-exchange column chromatography. All of them yield essentially pure T7 RNAP with high specific activity." @default.
• W1994771235 created "2016-06-24" @default.
• W1994771235 creator A5050838462 @default.
• W1994771235 creator A5057885946 @default.
• W1994771235 date "1998-11-01" @default.
• W1994771235 modified "2023-09-27" @default.
• W1994771235 title "Pseudo-Affinity Column Chromatography Based Rapid Purification Procedure for T7 RNA Polymerase" @default.
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• W1994771235 doi "https://doi.org/10.1080/10826069808010146" @default.
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