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• W1994776263 abstract "DAO Diseases of Aquatic Organisms Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout the JournalEditorsSpecials DAO 60:241-246 (2004) - doi:10.3354/dao060241 Establishment of a continuous embryonic cell line from Japanese flounder Paralichthys olivaceus for virus isolation Song-Lin Chen1,*, Guo-Cheng Ren1,2, Zhen-Xia Sha1, Cheng-Yin Shi1 1Yellow Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences, Qingdao 266071, PR China2College of Marine Life Science, China Ocean University, Qingdao 266003, PR China *Email: chensl@ysfri.ac.cn ABSTRACT: A continuous cell line, the flounder embryonic cell line (FEC), was established from gastrula-stage embryos of a marine cultured fish, the Japanese flounder Paralichthys olivaceus and cultured for more than 200 d with more than 60 passages. FEC cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with antibiotics, fetal bovine serum (FBS), sea perch serum (SPS), and basic fibroblast growth factor (bFGF). The cells were small and round, and grew actively and stably in culture. The effect of temperature, FBS concentration and bFGF on FEC cell growth was examined. Cells grew well between 24 and 30°C, but had a reduced growth rate below 18°C. The growth rate of FEC cells in medium containing 15% FBS was higher than that in medium containing 7.5% FBS. Addition of bFGF to the medium also significantly increased the growth rate. Chromosome analysis revealed that FEC cells have a normal diploid karyotype with 2n = 48. High survival rate was obtained after cryopreservation of cell cultures. The susceptibility of the cell line to piscine viruses was examined. Two viruses tested were shown to induce CPE (cytopathic effect) on FEC cells. FEC cell culture infected with fish iridovirus was further elucidated by electron microscopy. Many virus particles were found in the cytoplasm of the virus-infected FEC cells. These results indicated that the FEC cell line could be potentially used to isolate and study fish viruses. KEY WORDS: Embryonic cell line · Flounder · Paralichthys olivaceus · Virus · Susceptibility Full article in pdf format PreviousNextExport citation RSS - Facebook - Tweet - linkedIn Cited by Published in DAO Vol. 60, No. 3. Online publication date: September 08, 2004 Print ISSN: 0177-5103; Online ISSN: 1616-1580 Copyright © 2004 Inter-Research." @default.
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• W1994776263 title "Establishment of a continuous embryonic cell line from Japanese flounder Paralichthys olivaceus for virus isolation" @default.
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• W1994776263 doi "https://doi.org/10.3354/dao060241" @default.
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