FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1994800735> ?p ?o ?g. }

• W1994800735 endingPage "147" @default.
• W1994800735 startingPage "139" @default.
• W1994800735 abstract "Antigen-specific lymphocytes are important in the immune response to viral infection. Peripheral blood mononuclear cells (PBMC) are traditionally used as a source of effector cells in most immunological studies. We described here the use of the bispecific monoclonal antibodies (BSMAB) anti CD3:CD8 (CD3,8) and anti CD3:CD4 (CD3,4B) to expand and selectively enrich CD4+ and CD8+ T cells populations, respectively. The expanded cells demonstrated >90% CD3+CD4+ or CD3+CD8+ by 14 days. We measured HIV- and CMV-specific responses of these subset-enriched T cell and found that sensitivity and specificity is similar or higher when compared to PBMC in various cellular immunology assays (CMI). Vbeta analysis of BSMAB-enriched cells demonstrated comparable repertoire to the parent PBMC. Although both CD45RAhi and CD45ROhi cell populations were expanded with the BSMAB, selective subset depletion demonstrated that the antigen-specific T cell responses were restricted to the initial CD45ROhi memory effector subgroup. In conclusion, BSMAB in vitro enrichment of T cells allows significant expansion of the cell population without loss of specificity. This technique of cell expansion permits studies of T cell subset function in situations where the initial cell source is scarce, and presents an alternative for viable and functional T cells in immunological assays." @default.
• W1994800735 created "2016-06-24" @default.
• W1994800735 creator A5015818706 @default.
• W1994800735 creator A5025717970 @default.
• W1994800735 creator A5036075930 @default.
• W1994800735 creator A5066990530 @default.
• W1994800735 creator A5086948042 @default.
• W1994800735 creator A5087165714 @default.
• W1994800735 creator A5091027436 @default.
• W1994800735 date "2003-03-01" @default.
• W1994800735 modified "2023-10-03" @default.
• W1994800735 title "Evaluation of antigen-specific responses using in vitro enriched T cells" @default.
• W1994800735 cites W1508314184 @default.
• W1994800735 cites W1510551622 @default.
• W1994800735 cites W1932388426 @default.
• W1994800735 cites W1967075274 @default.
• W1994800735 cites W2037922983 @default.
• W1994800735 cites W2041746925 @default.
• W1994800735 cites W2107755945 @default.
• W1994800735 cites W2112633556 @default.
• W1994800735 cites W2119232577 @default.
• W1994800735 cites W2121372003 @default.
• W1994800735 cites W2127421167 @default.
• W1994800735 cites W2141782947 @default.
• W1994800735 cites W2163829877 @default.
• W1994800735 cites W2325887155 @default.
• W1994800735 doi "https://doi.org/10.1016/s0022-1759(02)00510-0" @default.
• W1994800735 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12609540" @default.
• W1994800735 hasPublicationYear "2003" @default.
• W1994800735 type Work @default.
• W1994800735 sameAs 1994800735 @default.
• W1994800735 citedByCount "35" @default.
• W1994800735 countsByYear W19948007352012 @default.
• W1994800735 countsByYear W19948007352014 @default.
• W1994800735 countsByYear W19948007352015 @default.
• W1994800735 countsByYear W19948007352016 @default.
• W1994800735 countsByYear W19948007352017 @default.
• W1994800735 countsByYear W19948007352020 @default.
• W1994800735 countsByYear W19948007352021 @default.
• W1994800735 countsByYear W19948007352022 @default.
• W1994800735 countsByYear W19948007352023 @default.
• W1994800735 crossrefType "journal-article" @default.
• W1994800735 hasAuthorship W1994800735A5015818706 @default.
• W1994800735 hasAuthorship W1994800735A5025717970 @default.
• W1994800735 hasAuthorship W1994800735A5036075930 @default.
• W1994800735 hasAuthorship W1994800735A5066990530 @default.
• W1994800735 hasAuthorship W1994800735A5086948042 @default.
• W1994800735 hasAuthorship W1994800735A5087165714 @default.
• W1994800735 hasAuthorship W1994800735A5091027436 @default.
• W1994800735 hasConcept C114684123 @default.
• W1994800735 hasConcept C137061746 @default.
• W1994800735 hasConcept C147483822 @default.
• W1994800735 hasConcept C153911025 @default.
• W1994800735 hasConcept C154317977 @default.
• W1994800735 hasConcept C159654299 @default.
• W1994800735 hasConcept C167672396 @default.
• W1994800735 hasConcept C202751555 @default.
• W1994800735 hasConcept C203014093 @default.
• W1994800735 hasConcept C2776090121 @default.
• W1994800735 hasConcept C2908647359 @default.
• W1994800735 hasConcept C2992881475 @default.
• W1994800735 hasConcept C51785407 @default.
• W1994800735 hasConcept C542903549 @default.
• W1994800735 hasConcept C54355233 @default.
• W1994800735 hasConcept C71924100 @default.
• W1994800735 hasConcept C86803240 @default.
• W1994800735 hasConcept C8891405 @default.
• W1994800735 hasConcept C99454951 @default.
• W1994800735 hasConceptScore W1994800735C114684123 @default.
• W1994800735 hasConceptScore W1994800735C137061746 @default.
• W1994800735 hasConceptScore W1994800735C147483822 @default.
• W1994800735 hasConceptScore W1994800735C153911025 @default.
• W1994800735 hasConceptScore W1994800735C154317977 @default.
• W1994800735 hasConceptScore W1994800735C159654299 @default.
• W1994800735 hasConceptScore W1994800735C167672396 @default.
• W1994800735 hasConceptScore W1994800735C202751555 @default.
• W1994800735 hasConceptScore W1994800735C203014093 @default.
• W1994800735 hasConceptScore W1994800735C2776090121 @default.
• W1994800735 hasConceptScore W1994800735C2908647359 @default.
• W1994800735 hasConceptScore W1994800735C2992881475 @default.
• W1994800735 hasConceptScore W1994800735C51785407 @default.
• W1994800735 hasConceptScore W1994800735C542903549 @default.
• W1994800735 hasConceptScore W1994800735C54355233 @default.
• W1994800735 hasConceptScore W1994800735C71924100 @default.
• W1994800735 hasConceptScore W1994800735C86803240 @default.
• W1994800735 hasConceptScore W1994800735C8891405 @default.
• W1994800735 hasConceptScore W1994800735C99454951 @default.
• W1994800735 hasIssue "1-2" @default.
• W1994800735 hasLocation W19948007351 @default.
• W1994800735 hasLocation W19948007352 @default.
• W1994800735 hasOpenAccess W1994800735 @default.
• W1994800735 hasPrimaryLocation W19948007351 @default.
• W1994800735 hasRelatedWork W1505145311 @default.
• W1994800735 hasRelatedWork W1513178816 @default.
• W1994800735 hasRelatedWork W1926032972 @default.
• W1994800735 hasRelatedWork W1978296203 @default.
• W1994800735 hasRelatedWork W2018916009 @default.
• W1994800735 hasRelatedWork W2029592815 @default.

• next