Matches in SemOpenAlex for { <https://semopenalex.org/work/W1994842793> ?p ?o ?g. }
Showing items 1 to 83 of
83
with 100 items per page.
- W1994842793 endingPage "e304" @default.
- W1994842793 startingPage "e304" @default.
- W1994842793 abstract "Background: Photodynamic therapy utilizes a photosensitizer, which upon excitation, emits energy that could be transduced by surrounding molecular oxygen to produce radical oxygen species. Interaction between the singlet oxygen species and viruses may have a virucidal effect. In this study, the potential of using a novel photodynamic system to inactivate enveloped virus was investigated. Methods: The photosensitizer used was zinc-phthalocyanine (ZnPC), which was absorbed onto a novel two-photon nanotransducer (NaYF4) that is activated by near infrared light (NIR). Dengue 2 virus was mixed with different concentrations of nanoparticles and exposed to a NIR light source at varying distances (5 cm and 10 cm) and durations (5 minutes and 10 minutes). The amount of surviving viruses was analyzed using plaque assay and compared with untreated virus samples. Further photo-inactivation study is being done on dengue 2 virus-infected HepG2 cells using nanoparticles that were conjugated with antibodies specific for the dengue virus envelope protein. The infected cells were incubated with the antibody-conjugated nanoparticles (4 to 110 μg/mL) and subjected to NIR illumination (10 cm for 5 minutes). Results: The level of virus inactivation increased with higher nanoparticle concentrations and at shorter distance and longer illumination from the NIR source. At all conditions, virus inactivation was complete when 2.2 mg/mL nanoparticles were used and at 44 μg/mL, virus titer was reduced by more than 50%. Conjugation with antibody may enhance the localization of the photo-inactivation site to targeted viruses or to virus-infected cells only, as shown by electron and laser scanning confocal microscopy. In addition, cell viability (MTT) assay showed that the photo-bleaching effect by NIR light on HepG2 cells was only minimal. Conclusion: Given its effectiveness in eradicating virus activity and its non-cytotoxic effect on cells, photodynamic therapy using NIR light could be a feasible novel technology with important implications in treating virus-infected blood or cellular samples." @default.
- W1994842793 created "2016-06-24" @default.
- W1994842793 creator A5013602233 @default.
- W1994842793 creator A5037964704 @default.
- W1994842793 creator A5063103444 @default.
- W1994842793 creator A5072226940 @default.
- W1994842793 creator A5078732727 @default.
- W1994842793 date "2008-12-01" @default.
- W1994842793 modified "2023-10-11" @default.
- W1994842793 title "Development of Novel Photo-inactivation Nanotechnology against Dengue Virus" @default.
- W1994842793 doi "https://doi.org/10.1016/j.ijid.2008.05.814" @default.
- W1994842793 hasPublicationYear "2008" @default.
- W1994842793 type Work @default.
- W1994842793 sameAs 1994842793 @default.
- W1994842793 citedByCount "1" @default.
- W1994842793 countsByYear W19948427932015 @default.
- W1994842793 crossrefType "journal-article" @default.
- W1994842793 hasAuthorship W1994842793A5013602233 @default.
- W1994842793 hasAuthorship W1994842793A5037964704 @default.
- W1994842793 hasAuthorship W1994842793A5063103444 @default.
- W1994842793 hasAuthorship W1994842793A5072226940 @default.
- W1994842793 hasAuthorship W1994842793A5078732727 @default.
- W1994842793 hasBestOaLocation W19948427931 @default.
- W1994842793 hasConcept C112613896 @default.
- W1994842793 hasConcept C12554922 @default.
- W1994842793 hasConcept C155672457 @default.
- W1994842793 hasConcept C159047783 @default.
- W1994842793 hasConcept C159654299 @default.
- W1994842793 hasConcept C171250308 @default.
- W1994842793 hasConcept C178790620 @default.
- W1994842793 hasConcept C185592680 @default.
- W1994842793 hasConcept C192562407 @default.
- W1994842793 hasConcept C203014093 @default.
- W1994842793 hasConcept C2522874641 @default.
- W1994842793 hasConcept C2777516457 @default.
- W1994842793 hasConcept C2779635636 @default.
- W1994842793 hasConcept C2781323092 @default.
- W1994842793 hasConcept C2781469039 @default.
- W1994842793 hasConcept C521977710 @default.
- W1994842793 hasConcept C533803919 @default.
- W1994842793 hasConcept C540031477 @default.
- W1994842793 hasConcept C75473681 @default.
- W1994842793 hasConcept C86803240 @default.
- W1994842793 hasConceptScore W1994842793C112613896 @default.
- W1994842793 hasConceptScore W1994842793C12554922 @default.
- W1994842793 hasConceptScore W1994842793C155672457 @default.
- W1994842793 hasConceptScore W1994842793C159047783 @default.
- W1994842793 hasConceptScore W1994842793C159654299 @default.
- W1994842793 hasConceptScore W1994842793C171250308 @default.
- W1994842793 hasConceptScore W1994842793C178790620 @default.
- W1994842793 hasConceptScore W1994842793C185592680 @default.
- W1994842793 hasConceptScore W1994842793C192562407 @default.
- W1994842793 hasConceptScore W1994842793C203014093 @default.
- W1994842793 hasConceptScore W1994842793C2522874641 @default.
- W1994842793 hasConceptScore W1994842793C2777516457 @default.
- W1994842793 hasConceptScore W1994842793C2779635636 @default.
- W1994842793 hasConceptScore W1994842793C2781323092 @default.
- W1994842793 hasConceptScore W1994842793C2781469039 @default.
- W1994842793 hasConceptScore W1994842793C521977710 @default.
- W1994842793 hasConceptScore W1994842793C533803919 @default.
- W1994842793 hasConceptScore W1994842793C540031477 @default.
- W1994842793 hasConceptScore W1994842793C75473681 @default.
- W1994842793 hasConceptScore W1994842793C86803240 @default.
- W1994842793 hasLocation W19948427931 @default.
- W1994842793 hasOpenAccess W1994842793 @default.
- W1994842793 hasPrimaryLocation W19948427931 @default.
- W1994842793 hasRelatedWork W2064628074 @default.
- W1994842793 hasRelatedWork W2127377584 @default.
- W1994842793 hasRelatedWork W2278717151 @default.
- W1994842793 hasRelatedWork W2560150159 @default.
- W1994842793 hasRelatedWork W2600642429 @default.
- W1994842793 hasRelatedWork W2897712569 @default.
- W1994842793 hasRelatedWork W2963416893 @default.
- W1994842793 hasRelatedWork W2965048408 @default.
- W1994842793 hasRelatedWork W3002932652 @default.
- W1994842793 hasRelatedWork W3127329048 @default.
- W1994842793 hasVolume "12" @default.
- W1994842793 isParatext "false" @default.
- W1994842793 isRetracted "false" @default.
- W1994842793 magId "1994842793" @default.
- W1994842793 workType "article" @default.