FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1994889800> ?p ?o ?g. }

• W1994889800 endingPage "e28707" @default.
• W1994889800 startingPage "e28707" @default.
• W1994889800 abstract "Cell-free protein expression (CFPE) comprised of in vitro transcription and translation is currently manipulated in relatively dilute solutions, in which the macromolecular crowding effects present in living cells are largely ignored. This may not only affect the efficiency of protein synthesis in vitro, but also limit our understanding of the functions and interactions of biomolecules involved in this fundamental biological process.Using cell-free synthesis of Renilla luciferase in wheat germ extract as a model system, we investigated the CFPE under macromolecular crowding environments emulated with three different crowding agents: PEG-8000, Ficoll-70 and Ficoll-400, which vary in chemical properties and molecular size. We found that transcription was substantially enhanced in the macromolecular crowding solutions; up to 4-fold increase in the mRNA production was detected in the presence of 20% (w/v) of Ficoll-70. In contrast, translation was generally inhibited by the addition of each of the three crowding agents. This might be due to PEG-induced protein precipitation and non-specific binding of translation factors to Ficoll molecules. We further explored a two-stage CFPE in which transcription and translation was carried out under high then low macromolecular crowding conditions, respectively. It produced 2.2-fold higher protein yield than the coupled CFPE control. The macromolecular crowding effects on CFPE were subsequently confirmed by cell-free synthesis of an approximately two-fold larger protein, Firefly luciferase, under macromolecular crowding environments.Three macromolecular crowding agents used in this research had opposite effects on transcription and translation. The results of this study should aid researchers in their choice of macromolecular crowding agents and shows that two-stage CFPE is more efficient than coupled CFPE." @default.
• W1994889800 created "2016-06-24" @default.
• W1994889800 creator A5007357474 @default.
• W1994889800 creator A5043401449 @default.
• W1994889800 creator A5048277748 @default.
• W1994889800 date "2011-12-08" @default.
• W1994889800 modified "2023-09-29" @default.
• W1994889800 title "Cell-Free Protein Expression under Macromolecular Crowding Conditions" @default.
• W1994889800 cites W1582365370 @default.
• W1994889800 cites W1587481053 @default.
• W1994889800 cites W1970948529 @default.
• W1994889800 cites W1974259661 @default.
• W1994889800 cites W1976205989 @default.
• W1994889800 cites W1980815840 @default.
• W1994889800 cites W1982214572 @default.
• W1994889800 cites W1984543814 @default.
• W1994889800 cites W1985876908 @default.
• W1994889800 cites W1992579668 @default.
• W1994889800 cites W1998284742 @default.
• W1994889800 cites W1999631164 @default.
• W1994889800 cites W1999703487 @default.
• W1994889800 cites W2001315574 @default.
• W1994889800 cites W2011358486 @default.
• W1994889800 cites W2015493085 @default.
• W1994889800 cites W2016081971 @default.
• W1994889800 cites W2017539509 @default.
• W1994889800 cites W2018832483 @default.
• W1994889800 cites W2026993723 @default.
• W1994889800 cites W2027913596 @default.
• W1994889800 cites W2030045395 @default.
• W1994889800 cites W2030260623 @default.
• W1994889800 cites W2030941532 @default.
• W1994889800 cites W2049294484 @default.
• W1994889800 cites W2051528354 @default.
• W1994889800 cites W2053099100 @default.
• W1994889800 cites W2057319782 @default.
• W1994889800 cites W2059139365 @default.
• W1994889800 cites W2060872711 @default.
• W1994889800 cites W2067618057 @default.
• W1994889800 cites W2072233846 @default.
• W1994889800 cites W2078862798 @default.
• W1994889800 cites W2080259922 @default.
• W1994889800 cites W2080803336 @default.
• W1994889800 cites W2082280554 @default.
• W1994889800 cites W2082301647 @default.
• W1994889800 cites W2083369386 @default.
• W1994889800 cites W2084016093 @default.
• W1994889800 cites W2084286730 @default.
• W1994889800 cites W2085881360 @default.
• W1994889800 cites W2087993212 @default.
• W1994889800 cites W2088858313 @default.
• W1994889800 cites W2089102241 @default.
• W1994889800 cites W2094701081 @default.
• W1994889800 cites W2096759266 @default.
• W1994889800 cites W2105452098 @default.
• W1994889800 cites W2107957687 @default.
• W1994889800 cites W2111497213 @default.
• W1994889800 cites W2113312001 @default.
• W1994889800 cites W2121553505 @default.
• W1994889800 cites W2131187223 @default.
• W1994889800 cites W2133773224 @default.
• W1994889800 cites W2134972813 @default.
• W1994889800 cites W2139161084 @default.
• W1994889800 cites W2147325150 @default.
• W1994889800 cites W2147700328 @default.
• W1994889800 cites W2147880580 @default.
• W1994889800 cites W2149378314 @default.
• W1994889800 cites W2150766477 @default.
• W1994889800 cites W2152821691 @default.
• W1994889800 cites W2158468016 @default.
• W1994889800 cites W2165375830 @default.
• W1994889800 cites W2165847215 @default.
• W1994889800 cites W4238701569 @default.
• W1994889800 doi "https://doi.org/10.1371/journal.pone.0028707" @default.
• W1994889800 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3234285" @default.
• W1994889800 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22174874" @default.
• W1994889800 hasPublicationYear "2011" @default.
• W1994889800 type Work @default.
• W1994889800 sameAs 1994889800 @default.
• W1994889800 citedByCount "90" @default.
• W1994889800 countsByYear W19948898002012 @default.
• W1994889800 countsByYear W19948898002013 @default.
• W1994889800 countsByYear W19948898002014 @default.
• W1994889800 countsByYear W19948898002015 @default.
• W1994889800 countsByYear W19948898002016 @default.
• W1994889800 countsByYear W19948898002017 @default.
• W1994889800 countsByYear W19948898002018 @default.
• W1994889800 countsByYear W19948898002019 @default.
• W1994889800 countsByYear W19948898002020 @default.
• W1994889800 countsByYear W19948898002021 @default.
• W1994889800 countsByYear W19948898002022 @default.
• W1994889800 countsByYear W19948898002023 @default.
• W1994889800 crossrefType "journal-article" @default.
• W1994889800 hasAuthorship W1994889800A5007357474 @default.
• W1994889800 hasAuthorship W1994889800A5043401449 @default.
• W1994889800 hasAuthorship W1994889800A5048277748 @default.
• W1994889800 hasBestOaLocation W19948898001 @default.
• W1994889800 hasConcept C10419127 @default.

• next