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• W1994914565 abstract "In RNA interference (RNAi), double-stranded RNA (dsRNA) triggers degradation of homologous messenger RNA. In many organisms, RNA-dependent RNA polymerase (RdRp) is required to initiate or amplify RNAi, but the substrate for dsRNA synthesis in vivo is not known. Here, we show that RdRp-dependent transgene silencing in Arabidopsis was caused by mutation of XRN4, which is a ribonuclease (RNase) implicated in mRNA turnover by means of decapping and 5′-3′ exonucleolysis. When both XRN4 and the RdRp were mutated, the plants accumulated decapped transgene mRNA. We propose that mRNAs lacking a cap structure become exposed to RdRp to initiate or maintain RNAi." @default.
• W1994914565 created "2016-06-24" @default.
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• W1994914565 date "2004-11-05" @default.
• W1994914565 modified "2023-10-16" @default.
• W1994914565 title "A Link Between mRNA Turnover and RNA Interference in <i>Arabidopsis</i>" @default.
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• W1994914565 doi "https://doi.org/10.1126/science.1101092" @default.
• W1994914565 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15528448" @default.
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