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- W1994920684 abstract "Prostaglandin (PG) specificity of two 3 alpha-hydroxysteroid/dihydrodiol dehydrogenase isozymes, DD2 and DD4, of human liver was examined. DD2 exhibited NADPH-linked reductase activity for 9-,11- and 15-ketoprostaglandins at a pH optimum of 6.0, whereas DD4 reduced only 15-ketoprostaglandin F2 alpha. DD2 showed the highest Vmax/Km value for PGD2 of the PG substrates, and the reduced product of PGD2 was identified to 9 alpha,11 beta-PGF2 by gas chromatography-mass spectrometry. In the reverse reaction with NADP+ as a cofactor, the two enzymes slowly oxidized several PGs with 9-, 11- and/or 15-hydroxy groups, except that DD2 showed high activity for 9 alpha,11 beta-PGF2 at a pH optimum of 10.0. The Km and Vmax values of DD2 for PGD2 were 57 microM and 250 nmol/min per mg, respectively, at pH 7.0 and 37 degrees C, and the respective values for 9 alpha,11 beta-PGF2 were 72 microM and 10 nmol/min per mg. PGD2 11-ketoreductase activity in human liver cytosol was recovered in 30-75% saturated ammonium sulfate fraction. More than 77% of the PGD2 11-ketoreductase activity in the ammonium sulfate fraction was immunoprecipitated by antibodies against DD2, and inhibited by known inhibitors of the enzyme. These results suggest that DD2 is a major soluble PGD2 11-ketoreductase species in human liver." @default.
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- W1994920684 date "1994-11-01" @default.
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- W1994920684 title "Reduction of prostaglandin D2 to 9α,11β-prostaglandin F2 by a human liver 3α-hydroxysteroid/dihydrodiol dehydrogenase isozyme" @default.
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- W1994920684 doi "https://doi.org/10.1016/0005-2760(94)90091-4" @default.
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