Matches in SemOpenAlex for { <https://semopenalex.org/work/W1994984189> ?p ?o ?g. }
Showing items 1 to 66 of
66
with 100 items per page.
- W1994984189 abstract "We are developing methods for random and site-specific labeling of individual DNA molecules to facilitate manipulation of fragments excised in the atomic force microscope (AFM) and for localization of specific DNA domains, such as protein binding sites and origins of replication. One successful method was to incorporate biotinylated nucleotides at random internal locations or specifically at the ends of linearized DNA molecules in vitro. Following complex formation with 5 nm diameter streptavidin-gold conjugates, chromatographic purification and passive adsorption of the complexes of mica, the biotinylated domains were easily localized in the AFM by virtue of the distinctive size and shape of the streptavidin-gold complex. In many cases unconjugated streptavidin (i.e., lacking gold) was also observed attached to the biotinylated DNA. A second approach to site-specific labeling of DNA for imaging in the AFM was to react DNA with restriction enzymes having sequence-specific binding properties. Like the unconjugated streptavidin-DNA complexes, these enzyme-DNA complexes were visible without attached colloidal gold. Efforts to image DNA labeled in vivo using bromodeoxyuridine (BrdU) and anti-BrdU antibodies are ongoing." @default.
- W1994984189 created "2016-06-24" @default.
- W1994984189 creator A5001550716 @default.
- W1994984189 creator A5014687168 @default.
- W1994984189 creator A5022243508 @default.
- W1994984189 creator A5039374612 @default.
- W1994984189 creator A5057590386 @default.
- W1994984189 date "1993-06-24" @default.
- W1994984189 modified "2023-09-23" @default.
- W1994984189 title "<title>Atomic force microscopy of DNA-colloidal gold and DNA-protein complexes</title>" @default.
- W1994984189 cites W1999288045 @default.
- W1994984189 cites W3150248096 @default.
- W1994984189 doi "https://doi.org/10.1117/12.146706" @default.
- W1994984189 hasPublicationYear "1993" @default.
- W1994984189 type Work @default.
- W1994984189 sameAs 1994984189 @default.
- W1994984189 citedByCount "3" @default.
- W1994984189 crossrefType "proceedings-article" @default.
- W1994984189 hasAuthorship W1994984189A5001550716 @default.
- W1994984189 hasAuthorship W1994984189A5014687168 @default.
- W1994984189 hasAuthorship W1994984189A5022243508 @default.
- W1994984189 hasAuthorship W1994984189A5039374612 @default.
- W1994984189 hasAuthorship W1994984189A5057590386 @default.
- W1994984189 hasBestOaLocation W19949841892 @default.
- W1994984189 hasConcept C104819515 @default.
- W1994984189 hasConcept C12554922 @default.
- W1994984189 hasConcept C155672457 @default.
- W1994984189 hasConcept C171250308 @default.
- W1994984189 hasConcept C185592680 @default.
- W1994984189 hasConcept C192562407 @default.
- W1994984189 hasConcept C204628709 @default.
- W1994984189 hasConcept C2776190903 @default.
- W1994984189 hasConcept C2778204606 @default.
- W1994984189 hasConcept C552990157 @default.
- W1994984189 hasConcept C55493867 @default.
- W1994984189 hasConcept C86803240 @default.
- W1994984189 hasConceptScore W1994984189C104819515 @default.
- W1994984189 hasConceptScore W1994984189C12554922 @default.
- W1994984189 hasConceptScore W1994984189C155672457 @default.
- W1994984189 hasConceptScore W1994984189C171250308 @default.
- W1994984189 hasConceptScore W1994984189C185592680 @default.
- W1994984189 hasConceptScore W1994984189C192562407 @default.
- W1994984189 hasConceptScore W1994984189C204628709 @default.
- W1994984189 hasConceptScore W1994984189C2776190903 @default.
- W1994984189 hasConceptScore W1994984189C2778204606 @default.
- W1994984189 hasConceptScore W1994984189C552990157 @default.
- W1994984189 hasConceptScore W1994984189C55493867 @default.
- W1994984189 hasConceptScore W1994984189C86803240 @default.
- W1994984189 hasLocation W19949841891 @default.
- W1994984189 hasLocation W19949841892 @default.
- W1994984189 hasOpenAccess W1994984189 @default.
- W1994984189 hasPrimaryLocation W19949841891 @default.
- W1994984189 hasRelatedWork W1970781522 @default.
- W1994984189 hasRelatedWork W1994984189 @default.
- W1994984189 hasRelatedWork W2001657816 @default.
- W1994984189 hasRelatedWork W2074057716 @default.
- W1994984189 hasRelatedWork W2095952308 @default.
- W1994984189 hasRelatedWork W2146428669 @default.
- W1994984189 hasRelatedWork W2396522900 @default.
- W1994984189 hasRelatedWork W2566515510 @default.
- W1994984189 hasRelatedWork W2716256600 @default.
- W1994984189 hasRelatedWork W3201188474 @default.
- W1994984189 isParatext "false" @default.
- W1994984189 isRetracted "false" @default.
- W1994984189 magId "1994984189" @default.
- W1994984189 workType "article" @default.