FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1994992737> ?p ?o ?g. }

• W1994992737 endingPage "5243" @default.
• W1994992737 startingPage "5243" @default.
• W1994992737 abstract "We characterize a novel fluorescence microscope which combines the high spatial discrimination of a total internal reflection epi-fluorescence (epi-TIRF) microscope with that of stimulated emission depletion (STED) nanoscopy. This combination of high axial confinement and dynamic-active lateral spatial discrimination of the detected fluorescence emission promises imaging and spectroscopy of the structure and function of cell membranes at the macro-molecular scale. Following a full theoretical description of the sampling volume and the recording of images of fluorescent beads, we exemplify the performance and limitations of the TIRF-STED nanoscope with particular attention to the polarization state of the laser excitation light. We demonstrate fluorescence correlation spectroscopy (FCS) with the TIRF-STED nanoscope by observing the diffusion of dye molecules in aqueous solutions and of fluorescent lipid analogs in supported lipid bilayers in the presence of background signal. The nanoscope reduced the out-of-focus background signal. A lateral resolution down to 40-50 nm was attained which was ultimately limited by the low lateral signal-to-background ratio inherent to the confocal epi-TIRF scheme. Together with the estimated axial confinement of about 55 nm, our TIRF-STED nanoscope achieved an almost isotropic and less than 1 attoliter small all-optically induced measurement volume." @default.
• W1994992737 created "2016-06-24" @default.
• W1994992737 creator A5019487894 @default.
• W1994992737 creator A5030151836 @default.
• W1994992737 creator A5067171744 @default.
• W1994992737 creator A5070786988 @default.
• W1994992737 creator A5088219208 @default.
• W1994992737 date "2012-02-17" @default.
• W1994992737 modified "2023-10-07" @default.
• W1994992737 title "Fluorescence correlation spectroscopy with a total internal reflection fluorescence STED microscope (TIRF-STED-FCS)" @default.
• W1994992737 cites W1539090868 @default.
• W1994992737 cites W1972160068 @default.
• W1994992737 cites W1972234131 @default.
• W1994992737 cites W1972259822 @default.
• W1994992737 cites W1976786825 @default.
• W1994992737 cites W1978249718 @default.
• W1994992737 cites W1979623029 @default.
• W1994992737 cites W1981765336 @default.
• W1994992737 cites W1981916955 @default.
• W1994992737 cites W1989011149 @default.
• W1994992737 cites W1991789201 @default.
• W1994992737 cites W1995689163 @default.
• W1994992737 cites W2010029433 @default.
• W1994992737 cites W2015512651 @default.
• W1994992737 cites W2022393754 @default.
• W1994992737 cites W2022912430 @default.
• W1994992737 cites W2033401401 @default.
• W1994992737 cites W2040435609 @default.
• W1994992737 cites W2047259287 @default.
• W1994992737 cites W2051542254 @default.
• W1994992737 cites W2052682847 @default.
• W1994992737 cites W2052994302 @default.
• W1994992737 cites W2054568006 @default.
• W1994992737 cites W2055252822 @default.
• W1994992737 cites W2056847422 @default.
• W1994992737 cites W2063255465 @default.
• W1994992737 cites W2064173118 @default.
• W1994992737 cites W2064757943 @default.
• W1994992737 cites W2068531027 @default.
• W1994992737 cites W2069621851 @default.
• W1994992737 cites W2089530043 @default.
• W1994992737 cites W2101036953 @default.
• W1994992737 cites W2115737236 @default.
• W1994992737 cites W2133054980 @default.
• W1994992737 cites W2137382396 @default.
• W1994992737 cites W2138597369 @default.
• W1994992737 cites W2141625497 @default.
• W1994992737 cites W2141802984 @default.
• W1994992737 cites W2149997985 @default.
• W1994992737 cites W2159501355 @default.
• W1994992737 cites W2161169163 @default.
• W1994992737 cites W2164857507 @default.
• W1994992737 cites W2167258646 @default.
• W1994992737 cites W2169791141 @default.
• W1994992737 cites W2170508051 @default.
• W1994992737 cites W2288750238 @default.
• W1994992737 doi "https://doi.org/10.1364/oe.20.005243" @default.
• W1994992737 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22418331" @default.
• W1994992737 hasPublicationYear "2012" @default.
• W1994992737 type Work @default.
• W1994992737 sameAs 1994992737 @default.
• W1994992737 citedByCount "60" @default.
• W1994992737 countsByYear W19949927372012 @default.
• W1994992737 countsByYear W19949927372013 @default.
• W1994992737 countsByYear W19949927372014 @default.
• W1994992737 countsByYear W19949927372015 @default.
• W1994992737 countsByYear W19949927372016 @default.
• W1994992737 countsByYear W19949927372017 @default.
• W1994992737 countsByYear W19949927372018 @default.
• W1994992737 countsByYear W19949927372019 @default.
• W1994992737 countsByYear W19949927372020 @default.
• W1994992737 countsByYear W19949927372021 @default.
• W1994992737 countsByYear W19949927372022 @default.
• W1994992737 countsByYear W19949927372023 @default.
• W1994992737 crossrefType "journal-article" @default.
• W1994992737 hasAuthorship W1994992737A5019487894 @default.
• W1994992737 hasAuthorship W1994992737A5030151836 @default.
• W1994992737 hasAuthorship W1994992737A5067171744 @default.
• W1994992737 hasAuthorship W1994992737A5070786988 @default.
• W1994992737 hasAuthorship W1994992737A5088219208 @default.
• W1994992737 hasBestOaLocation W19949927371 @default.
• W1994992737 hasConcept C105780059 @default.
• W1994992737 hasConcept C120665830 @default.
• W1994992737 hasConcept C121332964 @default.
• W1994992737 hasConcept C121381206 @default.
• W1994992737 hasConcept C144618238 @default.
• W1994992737 hasConcept C147080431 @default.
• W1994992737 hasConcept C169274487 @default.
• W1994992737 hasConcept C170395517 @default.
• W1994992737 hasConcept C185592680 @default.
• W1994992737 hasConcept C192562407 @default.
• W1994992737 hasConcept C30282128 @default.
• W1994992737 hasConcept C520434653 @default.
• W1994992737 hasConcept C67649825 @default.
• W1994992737 hasConcept C91881484 @default.
• W1994992737 hasConceptScore W1994992737C105780059 @default.
• W1994992737 hasConceptScore W1994992737C120665830 @default.
• W1994992737 hasConceptScore W1994992737C121332964 @default.

• next