FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1994996846> ?p ?o ?g. }

• W1994996846 endingPage "35794" @default.
• W1994996846 startingPage "35782" @default.
• W1994996846 abstract "Bacteria exploit multiple mechanisms for controlling central carbon metabolism (CCM). Thus, a bioinformatic analysis together with some experimental data implicated the HexR transcriptional factor as a global CCM regulator in some lineages of Gammaproteobacteria operating as a functional replacement of the Cra regulator characteristic of Enterobacteriales. In this study, we combined a large scale comparative genomic reconstruction of HexR-controlled regulons in 87 species of Proteobacteria with the detailed experimental analysis of the HexR regulatory network in the Shewanella oneidensis model system. Although nearly all of the HexR-controlled genes are associated with CCM, remarkable variations were revealed in the scale (from 1 to 2 target operons in Enterobacteriales up to 20 operons in Aeromonadales) and gene content of HexR regulons between 11 compared lineages. A predicted 17-bp pseudo-palindrome with a consensus tTGTAATwwwATTACa was confirmed as a HexR-binding motif for 15 target operons (comprising 30 genes) by in vitro binding assays. The negative effect of the key CCM intermediate, 2-keto-3-deoxy-6-phosphogluconate, on the DNA-regulator complex formation was verified. A dual mode of HexR action on various target promoters, repression of genes involved in catabolic pathways and activation of gluconeogenic genes, was for the first time predicted by the bioinformatic analysis and experimentally verified by changed gene expression pattern in S. oneidensis ΔhexR mutant. Phenotypic profiling revealed the inability of this mutant to grow on lactate or pyruvate as a single carbon source. A comparative metabolic flux analysis of wild-type and mutant strains of S. oneidensis using [(13)C]lactate labeling and GC-MS analysis confirmed the hypothesized HexR role as a master regulator of gluconeogenic flux from pyruvate via the transcriptional activation of phosphoenolpyruvate synthase (PpsA)." @default.
• W1994996846 created "2016-06-24" @default.
• W1994996846 creator A5006983657 @default.
• W1994996846 creator A5007293969 @default.
• W1994996846 creator A5009605432 @default.
• W1994996846 creator A5014224881 @default.
• W1994996846 creator A5023719427 @default.
• W1994996846 creator A5028603269 @default.
• W1994996846 creator A5041084611 @default.
• W1994996846 creator A5041157955 @default.
• W1994996846 creator A5084747098 @default.
• W1994996846 creator A5086861827 @default.
• W1994996846 date "2011-10-01" @default.
• W1994996846 modified "2023-10-10" @default.
• W1994996846 title "Control of Proteobacterial Central Carbon Metabolism by the HexR Transcriptional Regulator" @default.
• W1994996846 cites W1529622290 @default.
• W1994996846 cites W1552626264 @default.
• W1994996846 cites W1794923843 @default.
• W1994996846 cites W1931147290 @default.
• W1994996846 cites W1963812554 @default.
• W1994996846 cites W1965082990 @default.
• W1994996846 cites W1973751167 @default.
• W1994996846 cites W1983544779 @default.
• W1994996846 cites W1989277878 @default.
• W1994996846 cites W1999309464 @default.
• W1994996846 cites W2001077777 @default.
• W1994996846 cites W2007599192 @default.
• W1994996846 cites W2009874878 @default.
• W1994996846 cites W2010480471 @default.
• W1994996846 cites W2019122677 @default.
• W1994996846 cites W2035960476 @default.
• W1994996846 cites W2058529944 @default.
• W1994996846 cites W2070350400 @default.
• W1994996846 cites W2078151980 @default.
• W1994996846 cites W2078859290 @default.
• W1994996846 cites W2079048673 @default.
• W1994996846 cites W2090518060 @default.
• W1994996846 cites W2094789690 @default.
• W1994996846 cites W2103018940 @default.
• W1994996846 cites W2106533616 @default.
• W1994996846 cites W2107277218 @default.
• W1994996846 cites W2109940451 @default.
• W1994996846 cites W2114034054 @default.
• W1994996846 cites W2121892969 @default.
• W1994996846 cites W2132926880 @default.
• W1994996846 cites W2136070534 @default.
• W1994996846 cites W2148820057 @default.
• W1994996846 cites W2149833139 @default.
• W1994996846 cites W2152269861 @default.
• W1994996846 cites W2158266834 @default.
• W1994996846 cites W2158714788 @default.
• W1994996846 cites W2159774536 @default.
• W1994996846 cites W2159949023 @default.
• W1994996846 cites W2165335121 @default.
• W1994996846 cites W2166894101 @default.
• W1994996846 cites W4249435950 @default.
• W1994996846 doi "https://doi.org/10.1074/jbc.m111.267963" @default.
• W1994996846 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3195618" @default.
• W1994996846 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21849503" @default.
• W1994996846 hasPublicationYear "2011" @default.
• W1994996846 type Work @default.
• W1994996846 sameAs 1994996846 @default.
• W1994996846 citedByCount "46" @default.
• W1994996846 countsByYear W19949968462012 @default.
• W1994996846 countsByYear W19949968462013 @default.
• W1994996846 countsByYear W19949968462014 @default.
• W1994996846 countsByYear W19949968462015 @default.
• W1994996846 countsByYear W19949968462016 @default.
• W1994996846 countsByYear W19949968462017 @default.
• W1994996846 countsByYear W19949968462018 @default.
• W1994996846 countsByYear W19949968462019 @default.
• W1994996846 countsByYear W19949968462020 @default.
• W1994996846 countsByYear W19949968462021 @default.
• W1994996846 countsByYear W19949968462022 @default.
• W1994996846 crossrefType "journal-article" @default.
• W1994996846 hasAuthorship W1994996846A5006983657 @default.
• W1994996846 hasAuthorship W1994996846A5007293969 @default.
• W1994996846 hasAuthorship W1994996846A5009605432 @default.
• W1994996846 hasAuthorship W1994996846A5014224881 @default.
• W1994996846 hasAuthorship W1994996846A5023719427 @default.
• W1994996846 hasAuthorship W1994996846A5028603269 @default.
• W1994996846 hasAuthorship W1994996846A5041084611 @default.
• W1994996846 hasAuthorship W1994996846A5041157955 @default.
• W1994996846 hasAuthorship W1994996846A5084747098 @default.
• W1994996846 hasAuthorship W1994996846A5086861827 @default.
• W1994996846 hasBestOaLocation W19949968461 @default.
• W1994996846 hasConcept C101762097 @default.
• W1994996846 hasConcept C104317684 @default.
• W1994996846 hasConcept C143065580 @default.
• W1994996846 hasConcept C150194340 @default.
• W1994996846 hasConcept C203075996 @default.
• W1994996846 hasConcept C27153228 @default.
• W1994996846 hasConcept C2776989901 @default.
• W1994996846 hasConcept C2781170380 @default.
• W1994996846 hasConcept C42062724 @default.
• W1994996846 hasConcept C523546767 @default.
• W1994996846 hasConcept C54355233 @default.
• W1994996846 hasConcept C62361671 @default.

• next