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- W1995002594 abstract "Abstract A simple, rapid, and sensitive radioassay for N -acetylglucosamine-6-phosphate (GlcNAc-6-phosphate) deacetylase (EC 3.5.1.25) activity was developed in which the rate of production of [ 14 C]acetate was monitored. This assay was suitable for use in the presence of the interfering activity, phosphoacetylglucosamine mutase (EC 2.7.5.2). GlcNAc-6-phosphate deacetylase was induced by GlcNAc in Candida albicans and the cytosolic fraction prepared from these cells was employed as a source of enzyme. The K m for GlcNAc-6-phosphate was 1.0 m m . A method is described for the preparation of N -[ 14 C]acetylglucosamine 6-phosphate." @default.
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- W1995002594 date "1981-07-01" @default.
- W1995002594 modified "2023-09-23" @default.
- W1995002594 title "A rapid and sensitive assay for N-acetylglucosamine-6-phosphate deacetylase" @default.
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- W1995002594 doi "https://doi.org/10.1016/0003-2697(81)90517-0" @default.
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