FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1995004137> ?p ?o ?g. }

• W1995004137 endingPage "132" @default.
• W1995004137 startingPage "120" @default.
• W1995004137 abstract "Lentiviral vectors have gained much attention in recent years mainly because they integrate into nondividing host-cell genomes. For clinical applications, a safe and efficient lentiviral vector system is required. Previously, we have established a human immunodeficiency virus type 1 (HIV-1)-derived three-plasmid lentiviral vector system for viral vector production which includes a packaging vector pHP, a transducing vector pTV, and an envelope-encoding plasmid pHEF-VSVG. Cotransfection of these three plasmids into TE671 human rhabdomyosarcoma cells routinely yields 105–106 infectious units per milliliter in 24 h. Here we have extensively modified long terminal repeats (LTRs) of pTV to generate a safer lentiviral vector system. The 5′ U3 was replaced with a truncated cytomegalovirus (CMV) immediate early (IE) enhancer/TATA promoter and the 3′ U3 (except for the integration attachment site) was also deleted. These modifications resulted in a vector with 80% wild-type vector efficiency. Further deletion of 3′ U5 impaired vector function; however, this problem was solved by replacing the 3′ U5 with bovine growth hormone polyadenylation (bGHpA) sequence. The pTV vector containing all these modifications including the 5′ promoter substitution, the 3′ U3 deletion, and the substitution of 3′ U5 with bGHpA exhibited a self-inactivating (SIN) phenotype after transduction, transduced both dividing and nondividing cells at similar efficiencies, and produced vector titers twice as high as that of the wild-type construct. Thus, both safety and efficacy of the HP/TV vector have been improved by these LTR modifications. Further deletion of 5′ U5 impaired vector efficiency, suggesting that the 5′ U5 has critical roles in vector function." @default.
• W1995004137 created "2016-06-24" @default.
• W1995004137 creator A5036127131 @default.
• W1995004137 creator A5043198099 @default.
• W1995004137 creator A5063580873 @default.
• W1995004137 date "1999-08-01" @default.
• W1995004137 modified "2023-10-11" @default.
• W1995004137 title "Self-Inactivating Lentiviral Vectors with U3 and U5 Modifications" @default.
• W1995004137 cites W1488258440 @default.
• W1995004137 cites W1501620407 @default.
• W1995004137 cites W1505598199 @default.
• W1995004137 cites W1545240550 @default.
• W1995004137 cites W1566785044 @default.
• W1995004137 cites W1576746816 @default.
• W1995004137 cites W1598820738 @default.
• W1995004137 cites W1616824889 @default.
• W1995004137 cites W164972714 @default.
• W1995004137 cites W1659719610 @default.
• W1995004137 cites W1835972708 @default.
• W1995004137 cites W1954929088 @default.
• W1995004137 cites W1962839969 @default.
• W1995004137 cites W1976694988 @default.
• W1995004137 cites W1981354921 @default.
• W1995004137 cites W1981449360 @default.
• W1995004137 cites W1991669721 @default.
• W1995004137 cites W2006519535 @default.
• W1995004137 cites W2008512248 @default.
• W1995004137 cites W2009761723 @default.
• W1995004137 cites W2011460282 @default.
• W1995004137 cites W2011767474 @default.
• W1995004137 cites W2014662954 @default.
• W1995004137 cites W2030220702 @default.
• W1995004137 cites W2031809466 @default.
• W1995004137 cites W2080589169 @default.
• W1995004137 cites W2080924595 @default.
• W1995004137 cites W2082888399 @default.
• W1995004137 cites W2091092355 @default.
• W1995004137 cites W2097752168 @default.
• W1995004137 cites W2103286425 @default.
• W1995004137 cites W2104741425 @default.
• W1995004137 cites W2110688434 @default.
• W1995004137 cites W2125045982 @default.
• W1995004137 cites W2160741454 @default.
• W1995004137 cites W2167523976 @default.
• W1995004137 cites W2180795626 @default.
• W1995004137 cites W2320400608 @default.
• W1995004137 cites W265271866 @default.
• W1995004137 doi "https://doi.org/10.1006/viro.1999.9850" @default.
• W1995004137 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10441560" @default.
• W1995004137 hasPublicationYear "1999" @default.
• W1995004137 type Work @default.
• W1995004137 sameAs 1995004137 @default.
• W1995004137 citedByCount "176" @default.
• W1995004137 countsByYear W19950041372012 @default.
• W1995004137 countsByYear W19950041372013 @default.
• W1995004137 countsByYear W19950041372014 @default.
• W1995004137 countsByYear W19950041372015 @default.
• W1995004137 countsByYear W19950041372016 @default.
• W1995004137 countsByYear W19950041372017 @default.
• W1995004137 countsByYear W19950041372018 @default.
• W1995004137 countsByYear W19950041372019 @default.
• W1995004137 countsByYear W19950041372020 @default.
• W1995004137 countsByYear W19950041372021 @default.
• W1995004137 countsByYear W19950041372022 @default.
• W1995004137 countsByYear W19950041372023 @default.
• W1995004137 crossrefType "journal-article" @default.
• W1995004137 hasAuthorship W1995004137A5036127131 @default.
• W1995004137 hasAuthorship W1995004137A5043198099 @default.
• W1995004137 hasAuthorship W1995004137A5063580873 @default.
• W1995004137 hasBestOaLocation W19950041371 @default.
• W1995004137 hasConcept C104317684 @default.
• W1995004137 hasConcept C111936080 @default.
• W1995004137 hasConcept C141231307 @default.
• W1995004137 hasConcept C150194340 @default.
• W1995004137 hasConcept C15152581 @default.
• W1995004137 hasConcept C153911025 @default.
• W1995004137 hasConcept C159047783 @default.
• W1995004137 hasConcept C199491388 @default.
• W1995004137 hasConcept C22744801 @default.
• W1995004137 hasConcept C32470452 @default.
• W1995004137 hasConcept C40767141 @default.
• W1995004137 hasConcept C54355233 @default.
• W1995004137 hasConcept C55493867 @default.
• W1995004137 hasConcept C86803240 @default.
• W1995004137 hasConcept C92087593 @default.
• W1995004137 hasConceptScore W1995004137C104317684 @default.
• W1995004137 hasConceptScore W1995004137C111936080 @default.
• W1995004137 hasConceptScore W1995004137C141231307 @default.
• W1995004137 hasConceptScore W1995004137C150194340 @default.
• W1995004137 hasConceptScore W1995004137C15152581 @default.
• W1995004137 hasConceptScore W1995004137C153911025 @default.
• W1995004137 hasConceptScore W1995004137C159047783 @default.
• W1995004137 hasConceptScore W1995004137C199491388 @default.
• W1995004137 hasConceptScore W1995004137C22744801 @default.
• W1995004137 hasConceptScore W1995004137C32470452 @default.
• W1995004137 hasConceptScore W1995004137C40767141 @default.
• W1995004137 hasConceptScore W1995004137C54355233 @default.
• W1995004137 hasConceptScore W1995004137C55493867 @default.

• next