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• W1995084402 abstract "Purpose: Peptide-15 (P-15) is an analog of the cell-binding domain of collagen. P-15 has been shown to facilitate physiological process in a way similar to collagen, to serve as anchorage for cells, and to promote the binding, migration, and differentiation of cells. However, how P-15 alters osteoblast activity to promote bone formation is poorly understood. We therefore attempted to address this question by using microarray techniques to investigate the microRNA (miRNA) expression in osteoblasts exposed to P-15. Materials: The miRNA oligonucleotide microarray provides a novel method to carry out genome-wide miRNA profiling in human samples. By using miRNA microarrays containing 329 probe designed from human miRNA sequence, we identified in osteoblast-like cells line (MG-63) cul-tured with P-15 several miRNA whose expression is significantly modified. Results: We identified 11 up-regulated miRNA (i.e., mir-337, mir-15b, mir-377, mir-100, mir-148a, mir-125a, mir-199a, mir-221, mir-let-7d, mir-92, mir-23b) and six down-regulated miRNA (i.e., mir-422a, mir-19a, mir-224, mir-145, mir-22, mir-29a). Conclusion: The data reported are, to our knowledge, the first on translation regulation in osteoblasts exposed to P-15. They can be relevant to better understand the molecular mechanism of bone regeneration and can serve as a model for comparing other materials with similar clinical effects." @default.
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• W1995084402 date "2008-03-01" @default.
• W1995084402 modified "2023-10-14" @default.
• W1995084402 title "Peptide-15 Changes miRNA Expression in Osteoblast-Like Cells" @default.
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• W1995084402 doi "https://doi.org/10.1097/id.0b013e318166d182" @default.
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