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- W1995142425 abstract "The Bio-Rad Variant Hemoglobin testing system is an automated high performance liquid chromatography analyzer marketed with a beta-thalassemia short program to quantify Hbs F and A2, and assist in detecting Hbs A, S, D, C, and E. Although the two hemoglobins present in Hb H disease, Hb Bart's and Hb H, are separated by the system, they are not quantitated. In this study we modified the beta-thalassemia short program in order to facilitate quantitation of Hb Bart's and Hb H. Blood samples from 60 patients with Hb H disease, with various underlying genotypes, were studied. Analyses were performed on the day of blood collection or on hemolysates stored at -80 degrees C in cyanide or carbomonoxy forms. The mean sum of Hb Bart's and Hb H levels in all patients was found to be 12% (range 1.8-35%). Patients with nondeletional mutations (or association of alpha(0) deletion and nondeletional mutations) had notably higher Hb Bart's and Hb H levels when compared to patients with deletional genotypes." @default.
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- W1995142425 date "1999-01-01" @default.
- W1995142425 modified "2023-09-27" @default.
- W1995142425 title "Rapid and Accurate Quantitation of Hb Bart's and Hb H Using Weak Cation Exchange High Performance Liquid Chromatography: Correlation with the α-Thalassemia Genotype" @default.
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- W1995142425 doi "https://doi.org/10.3109/03630269909005700" @default.
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