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- W1995185612 abstract "Extracts prepared from the eyestalks of Uca pugilator were subjected to highvoltage vertical starch-gel electrophoresis to study the behavior of the melanindispersing fraction of the eyestalk extract in an electrical field. Separations were made in a pH range from 2.05 to 8.15 with McIlvaine buffer and at pH 8.9 with barbital buffer. During electrophoresis the apparatus was placed at about 3°C and an aluminum plate closely applied to the Saran-wrapped gel mold acted as a heat sink to dissipate the heat created in the gel. Over the range of hydrogen ion concentrations employed the biologically active fraction migrated as a single component. The isoelectric point appeared to be about pH 5.15. The hormonal activity migrated as much as 10 cm and responded in a manner predictable for an ampholyte to changes in voltage and pH. The assay procedure resolved the width of the active band within 1 or 2 cm of gel." @default.
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- W1995185612 date "1967-04-01" @default.
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- W1995185612 title "Characterization of the melanin-dispersing hormone fraction of the eyestalks of Uca pugilator by electrophoresis" @default.
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- W1995185612 doi "https://doi.org/10.1016/0016-6480(67)90067-6" @default.
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