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- W1995187513 abstract "A new method for measuring the rate of enzymatic excision of the carboxy-terminal, nonhelical fragment from type I procollagen is presented. Human procollagen containing [3H]tryptophan-labeled carboxy-terminal extensions was used as the substrate, and the enzyme was derived from the culture medium of mouse 3T6 fibroblasts. Incubation mixtures of substrate with enzyme were made 25% in ethanol which left the excised radiolabeled carboxy-terminal fragment in solution, whereas all other radiolabeled components were precipitated. Enzymatic activity was measured by radioactive counting of the ethanol supernatant. The assay is simple, rapid, sensitive and generates valid kinetic data." @default.
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- W1995187513 title "A method for assaying the activity of the endopeptidase which excises the nonhelical carboxyterminal extensions from type I procollagen" @default.
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- W1995187513 doi "https://doi.org/10.1016/0003-2697(78)90770-4" @default.
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