FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1995296768> ?p ?o ?g. }

• W1995296768 endingPage "1379" @default.
• W1995296768 startingPage "1373" @default.
• W1995296768 abstract "Epinephrine and other β-adrenergic receptor (βAR) agonists are often administered during cardiopulmonary resuscitation, a time when acid-base abnormalities and arrhythmias also commonly occur. We tested whether β2AR binding is influenced by pH or the antiarrhythmic drug lidocaine, and whether pH might influence the interaction of lidocaine with β2ARS. With institutional review board approval and informed consent, 32 venous blood samples were obtained from volunteers. Lymphocytes (which bear β2ARS similar to those found in heart) were isolated by density gradient centrifugation. Specific binding of the βAR ligand 3H-dihydroalprenolol (3H-DHA) was determined with lidocaine concentrations ranging from 10−6 to 10−2 mol/L (n=18 experiments), and with and without lidocaine (n=10 experiments), 100 pmol/L, and with and without QX314 (a permanently charged lidocaine derivative), 1 mmol/L (n=4 experiments). Data are presented as percent of control-specific binding measured at a pH of 7.4. Statistical analysis consisted of Spearman's rank-test. 3H-DHA-specific binding increased (p<.001) with pH. Thus, alkaline conditions favored binding of 3H-DHA to the receptor. Lidocaine inhibited 3H-DHA binding to β2ARS in a concentration-dependent manner. The concentration that inhibited specific binding of 3H-DHA by 50% was 3.1×10−4 mol/L (95% confidence limits, 1.3×10−4 to 7.5×10−4 mol/L). Lidocaine potency at inhibiting β2AR binding also increased with increasing pH; thus, there was limited benefit (in terms of increasing binding to β2ARS) to increasing pH when lidocaine was present. QX314, despite being present in a 10-fold greater concentration than lidocaine, had no effect on 3H-DHA binding at any tested pH. The affinity of β2ARS for both 3H-DHA and lidocaine increased with pH. Thus, the response to β2AR agonists (when no lidocaine is present) might be expected to be greater with normal or alkalotic pH than under acidotic conditions, supporting the correction of metabolic acidosis to achieve optimal effects from β2AR agonists during resuscitation. Epinephrine and other β-adrenergic receptor (βAR) agonists are often administered during cardiopulmonary resuscitation, a time when acid-base abnormalities and arrhythmias also commonly occur. We tested whether β2AR binding is influenced by pH or the antiarrhythmic drug lidocaine, and whether pH might influence the interaction of lidocaine with β2ARS. With institutional review board approval and informed consent, 32 venous blood samples were obtained from volunteers. Lymphocytes (which bear β2ARS similar to those found in heart) were isolated by density gradient centrifugation. Specific binding of the βAR ligand 3H-dihydroalprenolol (3H-DHA) was determined with lidocaine concentrations ranging from 10−6 to 10−2 mol/L (n=18 experiments), and with and without lidocaine (n=10 experiments), 100 pmol/L, and with and without QX314 (a permanently charged lidocaine derivative), 1 mmol/L (n=4 experiments). Data are presented as percent of control-specific binding measured at a pH of 7.4. Statistical analysis consisted of Spearman's rank-test. 3H-DHA-specific binding increased (p<.001) with pH. Thus, alkaline conditions favored binding of 3H-DHA to the receptor. Lidocaine inhibited 3H-DHA binding to β2ARS in a concentration-dependent manner. The concentration that inhibited specific binding of 3H-DHA by 50% was 3.1×10−4 mol/L (95% confidence limits, 1.3×10−4 to 7.5×10−4 mol/L). Lidocaine potency at inhibiting β2AR binding also increased with increasing pH; thus, there was limited benefit (in terms of increasing binding to β2ARS) to increasing pH when lidocaine was present. QX314, despite being present in a 10-fold greater concentration than lidocaine, had no effect on 3H-DHA binding at any tested pH. The affinity of β2ARS for both 3H-DHA and lidocaine increased with pH. Thus, the response to β2AR agonists (when no lidocaine is present) might be expected to be greater with normal or alkalotic pH than under acidotic conditions, supporting the correction of metabolic acidosis to achieve optimal effects from β2AR agonists during resuscitation." @default.
• W1995296768 created "2016-06-24" @default.
• W1995296768 creator A5035777364 @default.
• W1995296768 creator A5086479943 @default.
• W1995296768 date "1995-11-01" @default.
• W1995296768 modified "2023-10-07" @default.
• W1995296768 title "Effect of pH and Lidocaine on β-Adrenergic Receptor Binding" @default.
• W1995296768 cites W145271282 @default.
• W1995296768 cites W1884142619 @default.
• W1995296768 cites W1964171562 @default.
• W1995296768 cites W1976587269 @default.
• W1995296768 cites W1976597099 @default.
• W1995296768 cites W1977918428 @default.
• W1995296768 cites W1978784597 @default.
• W1995296768 cites W1980708881 @default.
• W1995296768 cites W1983523142 @default.
• W1995296768 cites W1988274287 @default.
• W1995296768 cites W1991335504 @default.
• W1995296768 cites W1991568720 @default.
• W1995296768 cites W1993721232 @default.
• W1995296768 cites W1993731818 @default.
• W1995296768 cites W1996140321 @default.
• W1995296768 cites W1998991340 @default.
• W1995296768 cites W1999858837 @default.
• W1995296768 cites W2014052704 @default.
• W1995296768 cites W2019159599 @default.
• W1995296768 cites W2019980095 @default.
• W1995296768 cites W2026748026 @default.
• W1995296768 cites W2037073620 @default.
• W1995296768 cites W2048513206 @default.
• W1995296768 cites W2051580385 @default.
• W1995296768 cites W2057590856 @default.
• W1995296768 cites W2066571203 @default.
• W1995296768 cites W2070262283 @default.
• W1995296768 cites W2072111883 @default.
• W1995296768 cites W2072749656 @default.
• W1995296768 cites W2078738419 @default.
• W1995296768 cites W2078821896 @default.
• W1995296768 cites W2080684842 @default.
• W1995296768 cites W2082104338 @default.
• W1995296768 cites W2118137246 @default.
• W1995296768 cites W2126169750 @default.
• W1995296768 cites W2132095149 @default.
• W1995296768 cites W2135809732 @default.
• W1995296768 cites W2168575394 @default.
• W1995296768 cites W2317555415 @default.
• W1995296768 cites W2338323241 @default.
• W1995296768 cites W2417466988 @default.
• W1995296768 cites W2425022801 @default.
• W1995296768 cites W4230154969 @default.
• W1995296768 cites W4245302739 @default.
• W1995296768 cites W4300648501 @default.
• W1995296768 cites W4327847211 @default.
• W1995296768 doi "https://doi.org/10.1378/chest.108.5.1373" @default.
• W1995296768 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7587445" @default.
• W1995296768 hasPublicationYear "1995" @default.
• W1995296768 type Work @default.
• W1995296768 sameAs 1995296768 @default.
• W1995296768 citedByCount "44" @default.
• W1995296768 countsByYear W19952967682012 @default.
• W1995296768 countsByYear W19952967682013 @default.
• W1995296768 countsByYear W19952967682014 @default.
• W1995296768 countsByYear W19952967682015 @default.
• W1995296768 countsByYear W19952967682016 @default.
• W1995296768 countsByYear W19952967682019 @default.
• W1995296768 countsByYear W19952967682021 @default.
• W1995296768 countsByYear W19952967682023 @default.
• W1995296768 crossrefType "journal-article" @default.
• W1995296768 hasAuthorship W1995296768A5035777364 @default.
• W1995296768 hasAuthorship W1995296768A5086479943 @default.
• W1995296768 hasConcept C126322002 @default.
• W1995296768 hasConcept C134018914 @default.
• W1995296768 hasConcept C170493617 @default.
• W1995296768 hasConcept C2780149897 @default.
• W1995296768 hasConcept C42219234 @default.
• W1995296768 hasConcept C71924100 @default.
• W1995296768 hasConcept C98274493 @default.
• W1995296768 hasConceptScore W1995296768C126322002 @default.
• W1995296768 hasConceptScore W1995296768C134018914 @default.
• W1995296768 hasConceptScore W1995296768C170493617 @default.
• W1995296768 hasConceptScore W1995296768C2780149897 @default.
• W1995296768 hasConceptScore W1995296768C42219234 @default.
• W1995296768 hasConceptScore W1995296768C71924100 @default.
• W1995296768 hasConceptScore W1995296768C98274493 @default.
• W1995296768 hasIssue "5" @default.
• W1995296768 hasLocation W19952967681 @default.
• W1995296768 hasLocation W19952967682 @default.
• W1995296768 hasOpenAccess W1995296768 @default.
• W1995296768 hasPrimaryLocation W19952967681 @default.
• W1995296768 hasRelatedWork W1967351389 @default.
• W1995296768 hasRelatedWork W1988206701 @default.
• W1995296768 hasRelatedWork W1997971171 @default.
• W1995296768 hasRelatedWork W2008744708 @default.
• W1995296768 hasRelatedWork W2011093127 @default.
• W1995296768 hasRelatedWork W2023156788 @default.
• W1995296768 hasRelatedWork W2064194781 @default.
• W1995296768 hasRelatedWork W2542010475 @default.
• W1995296768 hasRelatedWork W2748952813 @default.

• next